Choi S J, Meeran K, O'Shea D, Lambert P D, Bloom S R
Division of Endocrinology and Metabolism, Royal Postgraduate Medical School, Hammersmith Hospital, London, UK.
Brain Res. 1996 Aug 12;729(2):223-7. doi: 10.1016/s0006-8993(96)00423-4.
Cobalt protoporphyrin (CoPP) reduces food intake and body weight following intracerebroventricular (i.c.v.) administration in rats. We injected 0.2 mumol CoPP per kg body weight i.c.v. and monitored body weight and daily food intake for 7 days. The body weight and 24 h food intake of CoPP-treated animals was significantly lower than that of vehicle-treated animals in all studies (P < 0.01) from day 2 to day 7. The 2 h feeding response (CoPP vs. vehicle-treated) to 10 micrograms neuropeptide Y (NPY) (4.0 vs. 7.1 g; P < 0.05), the 1 h feeding response to 10 micrograms galanin (1.3 vs. 3.2 g; P < 0.05) and 30 micrograms norepinephrine (0.6 vs. 1.9 g; P < 0.05) in CoPP-treated animals were all reduced compared to the vehicle-treated group. In addition there was no change in hypothalamic NPY mRNA in CoPP-treated animals. I.c.v. CoPP decreases sensitivity to exogenous NPY, galanin and norepinephrine. The effect of CoPP is not specific to NPY as previously described.
在大鼠脑室内注射钴原卟啉（CoPP）后可减少食物摄入量和体重。我们以每千克体重0.2微摩尔的剂量给大鼠脑室内注射CoPP，并连续7天监测其体重和每日食物摄入量。在所有研究中，从第2天到第7天，接受CoPP治疗的动物的体重和24小时食物摄入量均显著低于接受赋形剂治疗的动物（P<0.01）。与接受赋形剂治疗的动物相比，接受CoPP治疗的动物对10微克神经肽Y（NPY）的2小时进食反应（CoPP组与赋形剂治疗组分别为4.0克对7.1克；P<0.05）、对10微克甘丙肽的1小时进食反应（1.3克对3.2克；P<0.05）以及对30微克去甲肾上腺素的进食反应（0.6克对1.9克；P<0.05）均降低。此外，接受CoPP治疗的动物下丘脑NPY mRNA没有变化。脑室内注射CoPP会降低对外源性NPY、甘丙肽和去甲肾上腺素的敏感性。CoPP的作用并不像之前所描述的那样对NPY具有特异性。
