CNRS, LAAS, 7 Avenue Du Colonel Roche, F-31400, Toulouse, France.
Laboratoire de Biologie Moléculaire Eucaryote (LBME), Centre de Biologie Intégrative (CBI), Université de Toulouse, CNRS, UPS, F-31062, Toulouse, France.
Biosens Bioelectron. 2021 Apr 15;178:112992. doi: 10.1016/j.bios.2021.112992. Epub 2021 Jan 27.
We present a fluorimetry-based technology for micro-RNA-21 (miR-21) sensing based on the concentration of miR-molecular beacon (MB) complexes and flushing of unbound MB. This concentration module consists of a microfluidic channel with the shape of a funnel operated with electrohydrodynamic actuation. We report a limit of detection of 2 pM in less than 1 min for miR-21 alone, and then demonstrate that miR-21 levels, measured in fine needle biopsy samples, from patients with pancreatic cancer correlate with the reference technique of reverse-transcription polymerase chain reaction (RT-PCR). Altogether, this technology has promising clinical performances for the follow-up of patients with cancer.
我们提出了一种基于荧光法的 microRNA-21（miR-21）传感技术，该技术基于 miR-分子信标（MB）复合物的浓度和未结合的 MB 的冲洗。该浓度模块由一个微流道组成，其形状为漏斗形，采用电动力学驱动。我们报告说，miR-21 单独的检测限为 2 pM，不到 1 分钟，然后证明从胰腺癌患者的细针活检样本中测量的 miR-21 水平与逆转录聚合酶链反应（RT-PCR）的参考技术相关。总的来说，这项技术在癌症患者的后续治疗中具有很有前景的临床应用。
