School of Pharmacy, Xi'an Jiaotong University, Xi'an, Shaanxi 710061, China; Institute of Vascular Materia Medica, Xi'an Jiaotong University, Xi'an, Shaanxi 710116, China.
School of Pharmacy, Xi'an Jiaotong University, Xi'an, Shaanxi 710061, China; Institute of Vascular Materia Medica, Xi'an Jiaotong University, Xi'an, Shaanxi 710116, China.
J Chromatogr A. 2021 Feb 22;1639:461916. doi: 10.1016/j.chroma.2021.461916. Epub 2021 Jan 22.
Cell membrane chromatography (CMC) is a biomimetic chromatographic method based on the ability of membrane receptors to selectively interact with their ligands in vivo. Using membrane receptors as a stationary phase, the CMC method helps in determining the binding characteristics between ligands and membrane receptors and in efficiently identifying specific target components in a complex sample that produce the cellular biological effects of ligands (drugs, antibodies, enzymes, cytokines, etc.). CMC is an analytical tool for revealing characteristics of ligand-receptor interactions, screening and discovering target substances, and accurately controlling the quality of drugs. Since establishment of CMC in the early 1990s, with the rapid development of cell biology, significant progress has been made in the development of high-expression receptors, engineered cell cultures, and standardized preparations, which allowed in vitro immobilization of cell membrane receptors and miniaturization of binding assays. A variety of CMC models have been established using different membrane receptors as a stationary phase, and many new methods have been developed by combining CMC with high-performance liquid chromatography (HPLC)/mass spectrometry or HPLC-IT-TOF technologies. CMC methods have been widely used to study drug-receptor interactions and to screen complex samples for effective or harmful components.
细胞膜色谱（CMC）是一种仿生色谱方法，基于膜受体在体内选择性地与配体相互作用的能力。该方法使用膜受体作为固定相，有助于确定配体与膜受体之间的结合特性，并有效地鉴定复杂样品中产生配体（药物、抗体、酶、细胞因子等）细胞生物学效应的特定靶成分。CMC 是一种分析工具，可揭示配体-受体相互作用的特征，筛选和发现靶物质，并准确控制药物的质量。自 20 世纪 90 年代初 CMC 建立以来，随着细胞生物学的快速发展，在高表达受体、工程细胞培养和标准化制剂的开发方面取得了重大进展，这使得细胞膜受体可以在体外固定化，并使结合测定小型化。使用不同的膜受体作为固定相建立了多种 CMC 模型，并通过将 CMC 与高效液相色谱（HPLC）/质谱或 HPLC-IT-TOF 技术相结合，开发了许多新方法。CMC 方法已广泛用于研究药物-受体相互作用，并筛选复杂样品中的有效或有害成分。
