Hou Xiaofang, Wang Sicen, Zhang Tao, Ma Jing, Zhang Jie, Zhang Yanmin, Lu Wen, He Huaizhen, He Langchong
Xi'an Jiaotong University Health Science Center, Yanta West Road, Xi'an 710061, Shaanxi, China.
Xi'an Jiaotong University Health Science Center, Yanta West Road, Xi'an 710061, Shaanxi, China.
J Pharm Biomed Anal. 2014 Dec;101:141-50. doi: 10.1016/j.jpba.2014.05.021. Epub 2014 May 23.
Traditional Chinese medicines (TCMs) have been used for preventative care for thousands of years. The active components are the basis for the pharmacodynamics of TCMs, and they can be an important source of lead compounds. As a bioaffinity chromatography technique, cell membrane chromatography (CMC) has been developed for almost 20 years since 1996. It has been proven to be a useful method for studying drug-receptor interactions and screening active components from medicinal herbs. In our review in 2007 (Drug Discov. Ther., 1 (2007) 104-107), the preparation, identification, evaluation, and preliminary applications of CMC stationary phases were presented. In this article, we briefly review some of the latest progress and applications about CMC including instrument development, research on drug-receptor interactions, screening active components from TCMs, and quality control of TCMs.
中药用于预防保健已有数千年历史。活性成分是中药药效学的基础，也是先导化合物的重要来源。细胞膜色谱（CMC）作为一种生物亲和色谱技术，自1996年以来已发展了近20年。它已被证明是研究药物-受体相互作用和从草药中筛选活性成分的有用方法。在我们2007年的综述（《药物发现与治疗》，2007年第1期，104 - 107页）中，介绍了CMC固定相的制备、鉴定、评价及初步应用。本文简要综述了CMC的一些最新进展和应用，包括仪器开发、药物-受体相互作用研究、从中药中筛选活性成分以及中药质量控制。
