College of Ecological Technology and Engineering, Shanghai Institute of Technology, Shanghai 201418, PR China.
State Key Laboratory of Pollution Control and Resource Reuse, Key Laboratory of Yangtze River Water Environment, Ministry of Education, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, PR China; Jiaxing Tongji Institute for Environment, Jiaxing, Zhejiang 314051, PR China; Shanghai Institute of Pollution Control and Ecological Security, Shanghai 200092, PR China.
Sci Total Environ. 2021 Jun 1;771:145315. doi: 10.1016/j.scitotenv.2021.145315. Epub 2021 Jan 21.
Antibiotics are gaining attention due to their roles as emerging pollutants and environmental obesogens, yet several aspects between their environmental exposure and obesogenic influence on organisms remain poorly explored. Here, Drosophila melanogaster were exposed to erythromycin (ERY, 0.1 μg/L) for three consecutive generations (F1 to F3). Body weight, circadian rhythm (represented by eclosion timing) and lipid metabolism were measured. ERY increased the size of lipid droplets in larvae of all three generations. It modestly inhibited body weight in adults that abnormally eclosed in the morning (AM adults) in the F1 and F2 generations, and the inhibition was less in adults that eclosed in the afternoon (PM adults). In contrast, it stimulated body weight in F3 adults. Notably, ERY promoted morning eclosion of females. Combining the effects from F1 to F3, acyl-CoA oxidase (ACO) was commonly increased in AM female and male adults and also in PM female ones, while it was commonly decreased in PM male adults. Glucokinase (GCK) was commonly increased in both sexes of AM adults but decreased in PM male adults across generations. The IIS pathway showed a common up-regulation in the AM adults despite some differences between sexes, but it did not show any shared changes in the PM adults with dysrhythmia. The AMPK pathway was involved across generations without particular shared changes. Collectively, the effects of ERY on the key metabolites and enzymes in glucolipid metabolism and the genetic regulations depended on sex, rhythm and exposure generation.
抗生素由于其作为新兴污染物和环境肥胖物的作用而受到关注，但它们在环境暴露和对生物体的肥胖影响之间的几个方面仍未得到充分探索。在这里，黑腹果蝇连续三代（F1 到 F3）暴露于红霉素（ERY，0.1μg/L）中。测量体重、昼夜节律（以羽化时间表示）和脂质代谢。ERY 增加了所有三代幼虫中脂滴的大小。它在 F1 和 F2 代中异常在早上（AM 成虫）羽化的成虫中适度抑制体重，而在下午（PM 成虫）羽化的成虫中抑制作用较小。相比之下，它刺激了 F3 代成虫的体重。值得注意的是，ERY 促进了雌性的早上羽化。将 F1 到 F3 的影响结合起来，酰基辅酶 A 氧化酶（ACO）在 AM 雌性和雄性成虫以及 PM 雌性成虫中普遍增加，而在 PM 雄性成虫中普遍减少。葡萄糖激酶（GCK）在 AM 成虫的两性中普遍增加，但在 F3 代的 PM 雄性成虫中减少。尽管两性之间存在一些差异，但 IIS 通路在 AM 成虫中表现出普遍的上调，但在节律异常的 PM 成虫中没有显示出任何共同的变化。AMPK 通路在几代中都有涉及，没有特别的共同变化。总的来说，ERY 对关键代谢物和酶在糖脂代谢中的作用以及遗传调控取决于性别、节律和暴露代。
