Department of Biochemistry, Government College University, Faisalabad, Pakistan.
Department of Applied Chemistry, Government College University, Faisalabad, Pakistan.
Plant Physiol Biochem. 2021 Mar;160:329-340. doi: 10.1016/j.plaphy.2021.01.037. Epub 2021 Jan 28.
Water-deficit stress is the most devastating environmental factor that adversely affects plant growth causing yield losses and low crop productivity. In this study, we employed sodium nitroprusside (SNP) as a seed priming agent for the acclimation of water-deficit stress in wheat plants by invoking priming memory.
The SNP-primed (75, 100, and 125 μM) and non-primed controls were allowed to grow in pots under water deficit and normal conditions. The flag leaves of 98-days mature plants were used for biochemical and physiological studies by following the well-established methods.
The antioxidant and hydrolytic enzymes were upregulated while reducing sugars, total sugars, and glycine betaine increased significantly in flag leaves of wheat plants originated from SNP-treated seeds compared to control under water deficit stress. However, a significant reduction in MDA and proline contents represented a lesser ROS production which resulted in enhanced cell membrane stability. Consequently, there was a significant enhancement in yield, plant biomass and 100 grains weight of wheat plants under water deficit stress.
The improvement in yield parameters indicates the induction of priming memory in SNP-primed seeds which elicit water deficit tolerance till the maturity of plants thus ensures sustainable productivity of wheat.
水分亏缺胁迫是影响植物生长的最具破坏性的环境因素，会导致产量损失和作物生产力降低。在这项研究中，我们采用硝普钠（SNP）作为引发剂，通过引发记忆来适应小麦植株的水分亏缺胁迫。
将 SNP 引发（75、100 和 125μM）和未引发的对照在水分亏缺和正常条件下的盆中生长。使用 98 天成熟植株的旗叶进行生化和生理研究，遵循已建立的方法。
与水分亏缺胁迫下的对照相比，来自 SNP 处理种子的小麦植株的旗叶中的抗氧化和水解酶上调，而还原糖、总糖和甘氨酸甜菜碱显著增加。然而，MDA 和脯氨酸含量的显著减少代表 ROS 产生减少，从而增强了细胞膜稳定性。因此，在水分亏缺胁迫下，小麦植株的产量、植物生物量和百粒重有显著提高。
产量参数的提高表明 SNP 引发种子中引发记忆的诱导，这种诱导可使小麦植株在成熟前保持水分亏缺耐受性，从而确保小麦的可持续生产力。
