Department of Applied Chemistry, Government College University, Faisalabad, Pakistan.
Department of Biochemistry, Government College University, Faisalabad, Pakistan.
BMC Plant Biol. 2022 Nov 22;22(1):540. doi: 10.1186/s12870-022-03912-2.
Around the globe, salinity is one of the serious environmental stresses which negatively affect rapid seed germination, uniform seedling establishment and plant developments restricting sustainable agricultural productivity. In recent years, the concepts of sustainable agriculture and cleaner production strategy have emphasized the introduction of greener agrochemicals using biocompatible and natural sources to maximize crop yield with minimum ecotoxicological effects. Over the last decade, the emergence of nanotechnology as a forefront of interdisciplinary science has introduced nanomaterials as fast-acting plant growth-promoting agents.
Herein, we report the preparation of nanocomposite using chitosan and green tea (CS-GTE NC) as an ecofriendly nanopriming agent to elicit salt stress tolerance through priming imprints. The CS-GTE NC-primed (0.02, 0.04 and 0.06%), hydroprimed and non-primed (control) wheat seeds were germinated under normal and salt stress (150 mM NaCl) conditions. The seedlings developed from aforesaid seeds were used for physiological, biochemical and germination studies. The priming treatments increased protein contents (10-12%), photosynthetic pigments (Chl a (4-6%), Chl b (34-36%), Total Chl (7-14%) and upregulated the machinery of antioxidants (CAT (26-42%), POD (22-43%)) in wheat seedlings under stress conditions. It also reduced MDA contents (65-75%) and regulated ROS production resulting in improved membrane stability. The priming-mediated alterations in biochemical attributes resulted in improved final germination (20-22%), vigor (4-11%) and germination index (6-13%) under both conditions. It reduced mean germination time significantly, establishing the stress-insulating role of the nanocomposite. The improvement of germination parameters validated the stimulation of priming memory in composite-treated seeds.
Pre-treatment of seeds with nanocomposite enables them to counter salinity at the seedling development stage by means of priming memory warranting sustainable plant growth and high crop productivity.
在全球范围内，盐度是一种严重的环境胁迫因素，它会对快速种子萌发、均匀幼苗建立和植物发育产生负面影响，从而限制可持续的农业生产力。近年来，可持续农业和清洁生产战略的概念强调引入生物相容性和天然来源的绿色农用化学品，以最大限度地提高作物产量，同时将生态毒性影响降至最低。在过去的十年中，纳米技术作为跨学科科学的前沿领域的出现，已经将纳米材料作为快速作用的植物生长促进剂。
本文报道了使用壳聚糖和绿茶（CS-GTE NC）作为一种环保的纳米引发剂来制备纳米复合材料，通过引发印记来引发耐盐胁迫。将 CS-GTE NC-引发（0.02、0.04 和 0.06%）、水引发和未引发（对照）的小麦种子在正常和盐胁迫（150 mM NaCl）条件下进行萌发。用上述种子发育的幼苗用于生理、生化和萌发研究。在胁迫条件下，引发处理增加了小麦幼苗中的蛋白质含量（10-12%）、光合色素（Chl a（4-6%）、Chl b（34-36%）、总 Chl（7-14%）和上调抗氧化剂的机械（CAT（26-42%）、POD（22-43%））。它还降低了 MDA 含量（65-75%）并调节了 ROS 产生，从而提高了膜稳定性。引发介导的生化特性的改变导致在两种条件下最终发芽率（20-22%）、活力（4-11%）和发芽指数（6-13%）得到改善。它显著缩短了平均发芽时间，确立了纳米复合材料的应激隔离作用。发芽参数的改善验证了复合处理种子中引发记忆的刺激作用。
种子的预处理使它们能够在幼苗发育阶段通过引发记忆来应对盐度，从而保证植物的可持续生长和高作物产量。
