Dahl Wendy J, Auger Jérémie, Alyousif Zainab, Miller Jennifer L, Tompkins Thomas A
Department of Food Science and Human Nutrition, University of Florida, 359 Newell Drive, Gainesville, FL, 32611, USA.
Rosell Institute for Microbiome and Probiotics, 6100 Royalmount, Montreal, QC, H4P 2R2, Canada.
BMC Res Notes. 2021 Feb 6;14(1):51. doi: 10.1186/s13104-021-05470-6.
Adults with Prader-Willi syndrome (PWS) require less energy intake to maintain body weight than the general adult population. This, combined with their altered gastrointestinal transit time, may impact microbiota composition. The aim of the study was to determine if the fecal microbiota composition of adults with PWS differed from non-affected adults. Using usual diet/non-interventional samples, fecal microbiota composition was analyzed using 16S rRNA gene amplicon sequencing and data from adults with PWS were merged with four other adult cohorts that differed by geographical location and age. QIIME 2™ sample-classifier, machine learning algorithms were used to cross-train the samples and predict from which dataset the taxonomic profiles belong. Taxa that most distinguished between all datasets were extracted and a visual inspection of the R library PiratePlots was performed to select the taxa that differed in abundance specific to PWS.
Fecal microbiota composition of adults with PWS showed low Blautia and enhanced RF39 (phyla Tenericutes), Ruminococcaceae, Alistipes, Erysipelotrichacaea, Parabacteriodes and Odoribacter. Higher abundance of Tenericutes, in particular, may be a signature characteristic of the PWS microbiota although its relationship, if any, to metabolic health is not yet known.
与一般成年人群相比，患有普拉德-威利综合征（PWS）的成年人维持体重所需的能量摄入较少。这一点，再加上他们改变的胃肠转运时间，可能会影响微生物群组成。本研究的目的是确定患有PWS的成年人的粪便微生物群组成是否与未受影响的成年人不同。使用常规饮食/非干预样本，通过16S rRNA基因扩增子测序分析粪便微生物群组成，并将来自患有PWS的成年人的数据与其他四个因地理位置和年龄不同的成年队列的数据合并。使用QIIME 2™样本分类器、机器学习算法对样本进行交叉训练，并预测分类学概况属于哪个数据集。提取所有数据集中最具区分性的分类群，并对R库PiratePlots进行目视检查，以选择PWS特有的丰度不同的分类群。
患有PWS的成年人的粪便微生物群组成显示布劳特氏菌属含量低，RF39（柔膜菌门）、瘤胃球菌科、嗜胆菌属、丹毒丝菌科、拟杆菌属和气味杆菌属含量增加。特别是，柔膜菌门的较高丰度可能是PWS微生物群的一个标志性特征，尽管其与代谢健康的关系（如果有的话）尚不清楚。
