Department of Food Science and Human Nutrition, University of Florida, Gainesville, FL, USA.
Division of Endocrinology, Department of Pediatrics, College of Medicine, University of Florida, Gainesville, FL, USA.
Mol Genet Genomic Med. 2020 Dec;8(12):e1535. doi: 10.1002/mgg3.1535. Epub 2020 Oct 25.
Probiotics may provide a benefit for adults with Prader-Willi syndrome (PWS) experiencing constipation. The primary aim was to determine if Bifidobacterium animalis ssp. lactis B94 (B. lactis B94) improves stool frequency, with secondary aims of stool form and gastrointestinal symptoms. Exploratory aims included diet quality and fecal microbiota composition.
Following a 4-week baseline, 25 adults with PWS were randomized to consume B. lactis B94 by capsule (15 billion) or placebo for 4 weeks, followed by 4-week washout in a double-blind, crossover design. Stool frequency and Bristol Stool Form (BSF) were assessed daily, and Gastrointestinal Symptom Rating Scale (GSRS) and dietary intake (7-days food records), per period. Fecal microbiota per period was analyzed using 16S rRNA gene amplicon sequencing and taxa of interest by qPCR (n = 24).
No adverse events were reported. Stool frequency at baseline (n = 25; 2.0 ± 0.1 stools/day), GSRS syndromes, and microbiota composition did not differ with the probiotic intervention overall; however, a delayed, carry-over effect on BSF types 6 and 7 was seen. Diet quality by HEI-2015 was 65.4 ± 8.5.
In adults with PWS, B. lactis B94 exhibited little effect on laxation over 4 weeks; however, further research is needed.
益生菌可能对患有普拉德-威利综合征（PWS）并伴有便秘的成年人有益。主要目的是确定双歧杆菌乳亚种 B94（B. lactis B94）是否能提高粪便频率，次要目的是评估粪便形态和胃肠道症状。探索性目的包括饮食质量和粪便微生物群落组成。
在 4 周基线期后，25 名 PWS 成年人随机分为益生菌组（服用 B. lactis B94 胶囊，剂量为 150 亿）或安慰剂组，各持续 4 周，然后进行双盲交叉洗脱期 4 周。每天评估粪便频率和布里斯托粪便形态（Bristol Stool Form，BSF），每个时期评估胃肠道症状评分量表（Gastrointestinal Symptom Rating Scale，GSRS）和饮食摄入量（7 天食物记录）。每个时期的粪便微生物群落均采用 16S rRNA 基因扩增子测序进行分析，并通过 qPCR 分析感兴趣的分类群（n=24）。
未报告不良事件。基线期（n=25；2.0±0.1 次/天）的粪便频率、GSRS 综合征和微生物群落组成在整个益生菌干预过程中均无差异；然而，BSF 类型 6 和 7 出现了延迟的、持续的效应。根据 HEI-2015 的饮食质量评分为 65.4±8.5。
在 PWS 成年人中，B. lactis B94 在 4 周内对排便的影响很小；然而，需要进一步研究。
