State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping 1, Lanzhou, Gansu, 730046, PR China.
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping 1, Lanzhou, Gansu, 730046, PR China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, 225009, PR China.
Ticks Tick Borne Dis. 2021 May;12(3):101677. doi: 10.1016/j.ttbdis.2021.101677. Epub 2021 Jan 27.
Anaplasma ovis, a tick-borne intra-erythrocytic Gram-negative bacterium, is a causative agent of ovine anaplasmosis. It is known that Dermacentor ticks act as biological vectors for A. ovis. VirD4 is the machine component of Type IV Secretion System of A. ovis. To better understand the pathogen-vector interaction, VirD4 was used as a bait protein for screening midgut proteins of Dermacentor silvarum via yeast two-hybrid mating assay. As a result, a ribosomal protein RL12 was identified from the midgut cDNA library of D. silvarum. For further validation, using in vitro Glutathione S-transferase (GST) pull-down assay, interaction between the proteins, GST-RL12 and HIS-VirD4, was observed in Western blot analysis. The study is first of its kind reporting a D. silvarum midgut protein interaction with VirD4 from A. ovis. Functional annotations showed some important cellular processes are attributed to the protein, particularly in the stringent response and biogenesis. The results of the study suggest the involvement of the VirD4-RL12 interaction in the regulation of signaling pathways, which is a tool for understanding the pathogen-vector interaction.
绵羊无浆体是一种寄生于红细胞内的革兰氏阴性蜱传细菌，是绵羊无浆体病的病原体。已知璃眼蜱属蜱是绵羊无浆体的生物传播媒介。VirD4 是绵羊无浆体 IV 型分泌系统的机器组件。为了更好地了解病原体-媒介物的相互作用，将 VirD4 用作酵母双杂交交配测定法筛选璃眼蜱属中肠蛋白的诱饵蛋白。结果从璃眼蜱属中肠 cDNA 文库中鉴定出一个核糖体蛋白 RL12。为了进一步验证，通过体外谷胱甘肽 S-转移酶（GST）下拉测定，在 Western blot 分析中观察到 GST-RL12 和 HIS-VirD4 之间的蛋白质相互作用。该研究首次报道了一种来自绵羊无浆体的璃眼蜱属中肠蛋白与 VirD4 的相互作用。功能注释表明，该蛋白与一些重要的细胞过程有关，特别是在严格反应和生物发生中。该研究结果表明，VirD4-RL12 相互作用参与了信号通路的调节，这是理解病原体-媒介物相互作用的一种工具。
