Guangzhou Key Laboratory of Subtropical Biodiversity and Biomonitoring, Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Sciences, South China Normal University, Guangzhou, 510631, China.
College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang, 524088, China.
Plant Physiol Biochem. 2021 Mar;160:365-376. doi: 10.1016/j.plaphy.2021.01.038. Epub 2021 Jan 28.
Theoretical and experimental studies have demonstrated that temperature is an important environmental factor that affects the regional distribution of plants. However, how to modify the distribution pattern of plants in different regions is a focus of current research. Obtain the information of cold tolerance genes from cold tolerance species, cloning genes with real cold tolerance effects is one of the most important ways to find the genes related to cold tolerance. In this study, we investigated whether transferring the VHA-c gene from Antarctic notothenioid fishes into Arabidopsis enhances freezing tolerance of Arabidopsis. The physiological response and molecular changes of VHA-c overexpressing pedigree and wildtype Arabidopsis were studied at -20 °C. The results showed that the malondialdehyde (MDA) and membrane leakage rates of WT plants were significantly higher than those of VHA-c8 and VHA-c11 plants, but the soluble sugar, soluble protein, proline and ATP contents of WT plants were significantly lower than those of VHA-c8 and VHA-c11 plants under -20 °C freezing treatment. The survival rate, VHA-c gene expression level and VHA-c protein contents of WT plants were significantly lower than those of VHA-c8 and VHA-c11 plants under -20 °C freezing treatment. Correlation analysis showed that ATP content was significantly negatively correlated with MDA and membrane leakage rate, and positively correlated with soluble sugar, soluble protein and proline content under -20 °C freezing treatment. These results demonstrated that overexpression of the VHA-c gene provided strong freezing tolerance to Arabidopsis by increasing the synthesis of ATP and improved the adaptability of plants in low temperature environment.
理论和实验研究表明，温度是影响植物区域分布的重要环境因素。然而，如何改变不同地区植物的分布模式是当前研究的重点。从耐寒物种中获取耐寒基因的信息，克隆具有真正耐寒效应的基因是寻找与耐寒性相关基因的最重要方法之一。在本研究中，我们研究了从南极鳕鱼中将 VHA-c 基因转入拟南芥是否增强拟南芥的抗冻性。在-20°C 下研究了 VHA-c 过表达系和野生型拟南芥的生理反应和分子变化。结果表明，在-20°C 冷冻处理下，WT 植物的丙二醛（MDA）和膜漏率明显高于 VHA-c8 和 VHA-c11 植物，但 WT 植物的可溶性糖、可溶性蛋白、脯氨酸和 ATP 含量明显低于 VHA-c8 和 VHA-c11 植物。在-20°C 冷冻处理下，WT 植物的存活率、VHA-c 基因表达水平和 VHA-c 蛋白含量明显低于 VHA-c8 和 VHA-c11 植物。相关性分析表明，在-20°C 冷冻处理下，ATP 含量与 MDA 和膜漏率呈显著负相关，与可溶性糖、可溶性蛋白和脯氨酸含量呈显著正相关。这些结果表明，通过增加 ATP 的合成，VHA-c 基因的过表达为拟南芥提供了很强的抗冻性，提高了植物在低温环境中的适应性。
