Centro de Ciências Rurais, Prédio 42, Departamento de Solos, Universidade Federal de Santa Maria, UFSM, Avenida Roraima n° 1000, Cidade Universitária, Bairro Camobi, Santa Maria, Rio Grande do Sul, CEP: 97105-900, Brazil.
UMR IC2MP 7285, CNRS/ Université de Poitiers, ENSIP, TSA41105, 1 rue Marcel Doré, 86073 Poitiers, Cedex 9, France.
Environ Sci Pollut Res Int. 2021 Jun;28(22):28808-28824. doi: 10.1007/s11356-020-11389-5. Epub 2021 Feb 6.
Active functional groups in biofilms determine the adsorption and desorption of contaminants and nutrients. Epilithic biofilms were characterized in order to understand the association between the chemistry alteration patterns and the surrounding anthropic activities of the Guaporé River watershed. The instrumental analyses included pyrolysis coupled to gas chromatography and mass spectroscopy, spectroscopy in the IR region with attenuated total reflectance, and two-dimensional nuclear magnetic resonance. Spectrometric techniques demonstrated that epilithic biofilms are mainly composed of polysaccharides, nitrogen-containing compounds, lipids, and aromatic and phenolic structures, which have functional groups characteristic of alcohols, esters, ethers, and amides. The polysaccharide levels reflect well the environmental pressures. The chemical composition of epilithic biofilms can be an effective tool for environmental assessment in watercourses, since the different anthropic actions developed in watersheds, mainly agriculture and urban areas, can modify the organic fraction of biofilms.
生物膜中的活性官能团决定了污染物和营养物质的吸附和解吸。为了了解瓜波雷河流域的化学变化模式与周围人为活动之间的关系，对附着生物膜进行了特征描述。仪器分析包括热解耦和气相色谱-质谱、衰减全反射红外光谱和二维核磁共振。光谱技术表明，附着生物膜主要由多糖、含氮化合物、脂质以及芳香族和酚类结构组成，这些结构具有醇、酯、醚和酰胺等官能团的特征。多糖水平很好地反映了环境压力。附着生物膜的化学成分可以成为河流环境评估的有效工具，因为流域内开展的不同人为活动，主要是农业和城市地区，会改变生物膜的有机部分。
