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山黧豆的植物化学成分和酶抑制潜力研究

Investigation of phytochemical composition and enzyme inhibitory potential of L.

机构信息

School of Pharmacy, Monash University Malaysia, Jalan Lagoon Selatan, Bandar Sunway, Selangor Darul Ehsan, Malaysia.

Institute of Pharmaceutical Sciences (IPS), University of Veterinary & Animal Sciences (UVAS), Lahore, Pakistan.

出版信息

Nat Prod Res. 2022 Jul;36(14):3750-3755. doi: 10.1080/14786419.2021.1880404. Epub 2021 Feb 8.

Abstract

L. commonly known as 'Scarlet Pimpernel' has been used in folklore as natural remedy for treating common ailments. The present research is aimed to explore the phytochemical composition and enzyme inhibition potential of methanol and dichloromethane (DCM) extracts of aerial and root parts. The phytochemical composition was established HPLC-PDA polyphenolic quantification and UHPLC-MS analysis, while the inhibition potential against amylase and tyrosinase enzymes were assessed using standard protocols. The HPLC-PDA polyphenolic quantification revealed the presence of important compounds including catechin, gallic acid, chlorogenic acid, and ferulic acid, whereas 34 different secondary metabolites were tentatively identified by UHPLC-MS of both the DCM extracts. All the extracts showed moderate tyrosinase and a weak amylase inhibition activity. The aerial-DCM extract showed comparatively higher tyrosinase and amylase enzyme inhibition potential, which may be due to the presence of secondary metabolites as tentatively identified by its UHPLC-MS profiling.

摘要

L. 俗称“红番花”,在民间医学中被用作治疗常见疾病的天然药物。本研究旨在探索甲醇和二氯甲烷(DCM)提取物对地上部分和根部分的植物化学成分组成和酶抑制潜力。通过 HPLC-PDA 多酚定量和 UHPLC-MS 分析确定了植物化学成分组成,同时采用标准方案评估了对淀粉酶和酪氨酸酶的抑制潜力。HPLC-PDA 多酚定量分析显示,存在重要的化合物,包括儿茶素、没食子酸、绿原酸和阿魏酸,而通过 DCM 提取物的 UHPLC-MS 分析,暂定鉴定了 34 种不同的次生代谢物。所有提取物均表现出中等的酪氨酸酶和较弱的淀粉酶抑制活性。地上部分-DCM 提取物显示出相对较高的酪氨酸酶和淀粉酶酶抑制潜力,这可能是由于其中存在的次生代谢物如通过其 UHPLC-MS 分析暂定鉴定的那样。

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