Mukai M, Ando K, Koike S
Hospital Division, National Institute of Radiological Sciences.
Gan To Kagaku Ryoho. 1988 Apr;15(4 Pt 1):631-5.
Effect of combination use of radiation and multiple administration of OK-432 was studied using a radio-resistant and weakly immunogenic murine fibrosarcoma (NFSa) originated spontaneously in a C3H female mouse. Mice implanted the tumor in the leg were locally irradiated with 40 Gy of gamma rays and locally administered total 8 KE of OK-432. The first group was given 1 KE daily for 8 days, 2 KE every other day in the 2nd group, 4 KE at 1 week interval in the 3rd group and 8 KE immediately after irradiation in the 4th group. The tumor volume at day 19 after irradiation was compared. The tumor growth inhibitory effect was observed in the 3rd group (p less than 0.01) and 4th group (p less than 0.05) compared to the group given radiation alone. This effect was seen at even more smaller dose of OK-432 as 0.5 KE by 2 times administration at 1 week interval. The effect of route of OK-432 administration was studied comparing the tumor volume at day 22. OK-432 was given 2 times at 1 week interval. A significant difference was seen in the group given OK-432 locally compared to the control group given radiation alone (p less than 0.001). There were no significant differences between the groups given OK-432 by the routes of i.p., s.c. i.v. and the control group. TCD50 value was examined giving OK-432 4 KE on the day and 2 KE on day 7 after irradiation. TCD50 value in the group treated with irradiation alone was 83.5 (79.6-87.4) Gy and the group given combination therapy with OK-432 was 60.7 (55.9-65.4) Gy. Two times OK-432 administration at 1 week interval appears to potentiate the radiation response by about 23 Gy.
使用源自C3H雌性小鼠自发产生的抗辐射且免疫原性较弱的鼠纤维肉瘤（NFSa），研究了放疗与多次注射OK-432联合使用的效果。将肿瘤接种于腿部的小鼠接受40 Gy的γ射线局部照射，并局部注射总量为8 KE的OK-432。第一组每天注射1 KE，共8天；第二组每隔一天注射2 KE；第三组每隔1周注射4 KE；第四组在照射后立即注射8 KE。比较照射后第19天的肿瘤体积。与单纯放疗组相比，第三组（p<0.01）和第四组（p<0.05）观察到肿瘤生长抑制作用。在以1周间隔分两次给予0.5 KE这种更小剂量的OK-432时也观察到了这种效果。通过比较第22天的肿瘤体积，研究了OK-432给药途径的效果。OK-432以1周间隔给药2次。与单纯放疗的对照组相比，局部给予OK-432的组有显著差异（p<0.001）。腹腔内、皮下、静脉内给予OK-432的组与对照组之间无显著差异。在照射当天给予4 KE、照射后第7天给予2 KE的情况下检测TCD50值。单纯放疗组的TCD50值为83.5（79.6 - 87.4）Gy，联合OK-432治疗组的TCD50值为60.7（55.9 - 65.4）Gy。以1周间隔两次给予OK-432似乎使辐射反应增强了约23 Gy。
