Yuan Maoxi, Yu Chunmei, Chen Xin, Wu Yubing
Department of Thoracic Surgery, Linyi Central Hospital, Linyi, China.
Department of Thoracic Surgery, The People's Hospital of Feixian County, Linyi, China.
Front Genet. 2021 Jan 21;11:610704. doi: 10.3389/fgene.2020.610704. eCollection 2020.
(small nuclear ribonucleoprotein polypeptide A) gene is essential for the pre-mRNA splicing process. Using the available datasets of TCGA or GEO, we aimed at exploring the potential association between the gene and lung cancer by several online tools (such as GEIPA2, MEXPRESS, Oncomine) and bioinformatics analysis software (R or GSEA). was highly expressed in the tissues of lung adenocarcinoma (LUAD) and lung squamous cell carcinoma tissue (LUSC), compared with control tissues. The high SNRPA expression was associated with a poor survival prognosis of LUAD cases, while the genetic alteration within was linked to the overall survival prognosis of LUSC cases. There was a potential correlation between promoter methylation and the expression of for LUAD. Compared with normal tissues, we observed a higher phosphorylation level at the S115 site of SNRPA protein (NP_004587.1) ( = 0.002) in the primary LUAD tissues. The potential ATR kinase of the S115 site was predicted. Besides, expression in lung cancer was negatively correlated with the infiltration level of M2 macrophage but positively correlated with that of Follicular B helper T cells, in both LUAD and LUSC. The enrichment analysis of -correlated genes showed that cell cycle and ubiquitin mechanism-related issues were mainly observed for LUAD; however, RNA splicing-related cellular issues were mainly for LUSC. In summary, the gene was identified as a potential prognosis biomarker of lung cancer, especially lung adenocarcinoma, which sheds new light on the association between the spliceosomal complex component and tumorigenesis.
（小核核糖核蛋白多肽A）基因对于前体mRNA剪接过程至关重要。利用TCGA或GEO的现有数据集，我们旨在通过几种在线工具（如GEIPA2、MEXPRESS、Oncomine）和生物信息学分析软件（R或GSEA）探索该基因与肺癌之间的潜在关联。与对照组织相比，该基因在肺腺癌（LUAD）和肺鳞状细胞癌组织（LUSC）中高表达。SNRPA高表达与LUAD病例的不良生存预后相关，而该基因内的基因改变与LUSC病例的总生存预后相关。LUAD的启动子甲基化与该基因的表达之间存在潜在相关性。与正常组织相比，我们在原发性LUAD组织中观察到SNRPA蛋白（NP_004587.1）的S115位点磷酸化水平更高（P = 0.002）。预测了S115位点的潜在ATR激酶。此外，在LUAD和LUSC中，该基因在肺癌中的表达与M2巨噬细胞的浸润水平呈负相关，但与滤泡辅助性B T细胞的浸润水平呈正相关。对该基因相关基因的富集分析表明，LUAD主要观察到细胞周期和泛素机制相关问题；然而，LUSC主要是RNA剪接相关的细胞问题。总之，该基因被鉴定为肺癌尤其是肺腺癌的潜在预后生物标志物，这为剪接体复合物成分与肿瘤发生之间的关联提供了新的线索。
