Interaction Between Dendritic Cells and β-Glucan Partially Depends on Dectin-1 and It Promotes High IL-10 Production by T Cells.

Host- interaction has been broadly studied during infection, with a progressive shift in focus toward non- species. is an emerging multidrug resistant pathogen causing rising morbidity and mortality worldwide. Therefore, understanding the interplay between the host immune system and is critically important. cell wall β-glucans play significant roles in the induction of host protective immune responses. However, it remains unclear how β-glucan impacts dendritic cell (DC) responses. In this study, we investigated DC maturation and function in response to β-glucans isolated from the cell walls of , , and . These three distinct β-glucans had differential effects on expression of the DC marker, CD11c, and on DC maturation. Furthermore, bone-marrow derived DCs (BMDCs) showed enhanced cytokine responses characterized by substantial interleukin (IL)-10 production following β-glucan stimulation. BMDCs stimulated with β-glucan augmented IL-10 production by T cells in tandem with increased IL-10 production by BMDCs. Inhibition of dectin-1 ligation demonstrated that the interactions between dectin-1 on DCs and cell wall β-glucans varied depending on the species. The effects of β-glucan were partially dependent on dectin-1, and this dependence, in part, led to distinct DC responses. Our study provides new insights into immune regulation by cell wall components. These data may be of use in the development of new clinical approaches for treatment of patients with infection.