Mehmood Rashid, Jibiki Kazuya, Shibazaki Noriko, Yasuhara Noriko
Department of Life Sciences, College of Science and General Studies, Alfaisal University, Riyadh, Kingdom of Saudi Arabia.
Graduate School of Integrated Basic Sciences, Nihon University, Setagaya-ku, Tokyo, Japan.
Heliyon. 2021 Jan 30;7(1):e06039. doi: 10.1016/j.heliyon.2021.e06039. eCollection 2021 Jan.
Transport of functional molecules across the nuclear membrane of a eukaryotic cell is regulated by a dedicated set of transporter proteins that carry molecules into the nucleus or out of the nucleus to the cytoplasm for homeostasis of the cell. One of the categories of cargo molecules these transporters carry are the molecules for cell cycle regulation. Therefore, their role is critical in terms of cancer development. Any misregulation of the transport factors would means aberrant abundance of cell cycle regulators and might have consequences in cell cycle progression. While earlier studies have focussed on individual transport related molecules, a collective overview of how these molecules may be dysregulated in breast cancer is lacking. Using genomic and transcriptomic datasets from TCGA (The Cancer Genome Atlas) and microarray platforms, we carried out bioinformatic analysis and provide a genetic and molecular profile of all the molecules directly related to nucleocytoplasmic shuttling of proteins and RNAs. Interestingly, we identified that many of these molecules are either mutated or have dysregulated expression in breast cancer. Strikingly, some of the molecules, namely, were correlated with poor patient survival. This study provides a comprehensive genetic and molecular landscape of nucleocytoplasmic factors in breast cancer and points to the important roles of various nucleocytoplasmic factors in cancer progression. This data might have implications in prognosis and therapeutic targeting in breast cancer.
真核细胞中功能性分子穿过核膜的过程，由一组专门的转运蛋白调控，这些转运蛋白将分子转运进细胞核或从细胞核转运至细胞质，以维持细胞的内稳态。这些转运蛋白所携带的一类货物分子是细胞周期调控分子。因此，它们在癌症发展方面的作用至关重要。转运因子的任何失调都意味着细胞周期调节因子的异常丰度，可能会对细胞周期进程产生影响。虽然早期研究聚焦于单个与转运相关的分子，但缺乏对这些分子在乳腺癌中可能如何失调的总体概述。利用来自TCGA（癌症基因组图谱）的基因组和转录组数据集以及微阵列平台，我们进行了生物信息学分析，并提供了所有与蛋白质和RNA核质穿梭直接相关的分子的遗传和分子概况。有趣的是，我们发现这些分子中的许多在乳腺癌中要么发生了突变，要么表达失调。引人注目的是，其中一些分子，即，与患者的不良生存相关。这项研究提供了乳腺癌中核质因子的全面遗传和分子图景，并指出了各种核质因子在癌症进展中的重要作用。这些数据可能对乳腺癌的预后和治疗靶点有影响。
