Biro G P, Taichman G C, Lada B, Keon W J, Rosen A L, Sehgal L R
Department of Physiology, School of Medicine, University of Ottawa, Ontario, Canada.
Artif Organs. 1988 Feb;12(1):40-50. doi: 10.1111/j.1525-1594.1988.tb01522.x.
The left anterior descending coronary artery was cannulated in anesthetized, open-chest dogs for use as a coronary test bed to assess the coronary vascular actions of oxygenated, unmodified and pyridoxylated, partially cross-linked (polymerized), stroma-free hemoglobin solutions (SFHS). The actions of these SFHSs were assessed in this test bed, by comparison with perfusion with whole blood. Unmodified SFHS caused significant vasoconstriction, whereas pyridoxylated, partially polymerized SFHS did not do so. The existing coronary flow during perfusion with either SFHS preparation, under basal conditions, did not increase during intracoronary infusion of adenosine. Coronary flow-autoregulation was also altered during perfusion with either SFHS, because the "normal" reactive hyperemia response, observed in the control experiments, was not seen. These findings suggest that both unmodified and pyridoxylated cross-linked (70% polymers) hemoglobin preparations possess some vascular activity when tested in the canine coronary circulation.
在麻醉开胸犬身上，将冠状动脉左前降支插管，用作冠状动脉试验台，以评估含氧的、未修饰的以及经吡哆醛化、部分交联（聚合）的无基质血红蛋白溶液（SFHS）的冠状血管作用。通过与全血灌注相比较，在该试验台中评估这些SFHS的作用。未修饰的SFHS引起显著的血管收缩，而经吡哆醛化、部分聚合的SFHS则不会。在基础条件下，使用任何一种SFHS制剂灌注时的现有冠脉血流量，在冠脉内输注腺苷期间并未增加。在用任何一种SFHS灌注期间，冠脉血流自动调节也发生了改变，因为在对照实验中观察到的“正常”反应性充血反应未出现。这些发现表明，在犬冠状动脉循环中进行测试时，未修饰的和经吡哆醛化交联（70%聚合物）的血红蛋白制剂均具有一定的血管活性。
