Lawless K, Anderson P J, Biro G P
Department of Biochemistry, University of Ottawa, Ontario, Canada.
Adv Exp Med Biol. 1989;248:365-70. doi: 10.1007/978-1-4684-5643-1_40.
Erythrocytes contain a high concentration of cytosolic adenosine deaminase and this enzyme activity is present in preparations of stroma-free hemoglobin (SFHS). The documented vasoconstrictor activity of SFHS preparations does not appear to be due to interference with endogenous adenosine-mediated mechanisms, because removal of greater than 80% of adenosine deaminase activity failed to affect the vasoconstrictor potency of SFHS preparations. We have also demonstrated the presence of a contractility-depressant activity in SFHS when the latter was added to aqueous buffer perfusing rat Langendorff-hearts. This activity is diminished in phosphocellulose-purified preparations with reduced adenosine deaminase activity but is not necessarily causally related to this enzyme.
红细胞含有高浓度的胞质腺苷脱氨酶，并且这种酶活性存在于无基质血红蛋白（SFHS）制剂中。SFHS制剂已记录的血管收缩活性似乎并非由于对内源性腺苷介导机制的干扰，因为去除超过80%的腺苷脱氨酶活性并未影响SFHS制剂的血管收缩效力。当将SFHS添加到灌注大鼠Langendorff心脏的水性缓冲液中时，我们还证明了SFHS中存在收缩抑制活性。这种活性在腺苷脱氨酶活性降低的磷酸纤维素纯化制剂中减弱，但不一定与该酶有因果关系。
