Huang Yu, Chen Jiarui, Lin Jin, Lin Jianhua, Chen Xuanwei
Department of Spine Surgery, The First Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian 350005, China.
Department of Pharmacy, Fujian Medical University, Fuzhou, Fujian 350108, China.
ACS Omega. 2021 Jan 19;6(4):2899-2905. doi: 10.1021/acsomega.0c05245. eCollection 2021 Feb 2.
Orthopedic infection is a serious complication in surgeries and remains a great challenge in clinics. Here, the natural antimicrobial compound vanillic acid-loaded gold nanospheres/mesoporous silica nanoparticles (VA@Au-MSNs) were fabricated for chemo-photothermal synergistic therapy to orthopedic infections. The shape and morphology of Au-MSN and VA@Au-MSN were observed by scanning electron microscopy and transmission electron microscopy. The properties of VA@Au-MSN or related components were characterized by dynamic light scattering, thermogravimetric analysis, Brunauer-Emmett-Teller (BET) analysis, and photothermal analysis. Vanillic acid released from VA@Au-MSN was detected in phosphate-buffered saline. A cytotoxicity test and an antibacterial assessment were performed to explore the biosafety and antibacterial activity of VA@Au-MSN, respectively. The results showed that Au-MSN possessed a high BET surface area (458 m/g). After loading vanillic acid, the BET surface area reduced to 72 m/g. The loading efficiency of Au-MSN was 18.56%. Under 808 nm laser irradiation, the temperature at the wound site injected with the Au-MSN solution in the mouse increased from 24 to 60 °C within about 12 s. Also, the high temperature could promote the release of vanillic acid from VA@Au-MSN. Additionally, VA@Au-MSN has no obvious cytotoxicity to MC3T3-E1 cells, but the generated local hyperthermia and the VA released from VA@Au-MSN had excellent antibacterial activity against in a synergistic way. In conclusion, the VA@Au-MSN with biosafety and excellent antibacterial performance might be applied for the treatment of orthopedic infection.
骨科感染是手术中的一种严重并发症，在临床上仍然是一个巨大的挑战。在此，制备了负载天然抗菌化合物香草酸的金纳米球/介孔二氧化硅纳米颗粒（VA@Au-MSNs）用于骨科感染的化学-光热协同治疗。通过扫描电子显微镜和透射电子显微镜观察Au-MSN和VA@Au-MSN的形状和形态。通过动态光散射、热重分析、布鲁诺尔-埃米特-泰勒（BET）分析和光热分析对VA@Au-MSN或相关成分的性质进行表征。在磷酸盐缓冲盐水中检测从VA@Au-MSN释放的香草酸。分别进行细胞毒性试验和抗菌评估以探索VA@Au-MSN的生物安全性和抗菌活性。结果表明，Au-MSN具有高BET表面积（458 m²/g）。负载香草酸后，BET表面积降至72 m²/g。Au-MSN的负载效率为18.56%。在808 nm激光照射下，小鼠伤口部位注射Au-MSN溶液后的温度在约12 s内从24℃升高到60℃。此外，高温可促进香草酸从VA@Au-MSN释放。另外，VA@Au-MSN对MC3T3-E1细胞没有明显细胞毒性，但产生的局部热疗和从VA@Au-MSN释放的VA具有协同的优异抗菌活性。总之，具有生物安全性和优异抗菌性能的VA@Au-MSN可能用于治疗骨科感染。
