结直肠癌小鼠模型：肿瘤和基质细胞在盲肠和直肠中的原位共植入。

Department of Pathology and Laboratory Medicine, Weill Cornell Medicine, New York, NY 10065, USA.

Department of Gastroenterological Surgery, Osaka City University Graduate School of Medicine, Osaka City 545-8585, Japan.

STAR Protoc. 2021 Jan 22;2(1):100297. doi: 10.1016/j.xpro.2021.100297. eCollection 2021 Mar 19.

interrogation of the functional role of genes implicated in colorectal cancer (CRC) is limited by the need for physiological models that mimic the disease. Here, we describe a protocol that provides the steps required for the orthotopic co-implantation of tumoral and stromal cells into the cecum and rectum to investigate the crosstalk between the tumor and its microenvironment. This protocol recapitulates metastases to the lymph nodes, liver, and lungs observed in human CRC. For complete details on the use and execution of this protocol, please refer to Kasashima et al. (2020).

探讨结直肠癌（CRC）相关基因的功能作用受到限制，因为需要模拟疾病的生理模型。在此，我们描述了一种将肿瘤细胞和基质细胞原位共植入盲肠和直肠以研究肿瘤与其微环境之间相互作用的方案。该方案再现了人 CRC 中观察到的淋巴结、肝和肺转移。如需详细了解该方案的使用和执行，请参考 Kasashima 等人（2020 年）的研究。