建立结直肠癌肝转移小鼠模型的方案。
Protocol to establish a mouse model for hepatic metastasis of colorectal cancer.
机构信息
State Key Laboratory of Cell Biology, Shanghai Key Laboratory of Molecular Andrology, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai 200031, China.
State Key Laboratory of Cell Biology, Shanghai Key Laboratory of Molecular Andrology, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai 200031, China.
出版信息
STAR Protoc. 2023 Mar 17;4(1):102023. doi: 10.1016/j.xpro.2022.102023. Epub 2023 Jan 12.
Hepatic metastasis is the leading cause of colorectal-cancer (CRC)-associated death. Here we describe an optimized protocol to establish a more clinically relevant mouse model of CRC metastasis. We detail steps for subcutaneous transplantation of luciferase-expressing CRC cells and subsequent orthotopic transplantation of subcutaneous tumor tissues. This mouse model allows CRC cells to form tumors within the intestinal tract and metastasize to the liver, thereby providing the approach to assess hepatic metastasis of CRC in vivo. For complete details on the use and execution of this protocol, please refer to Zhang et al. (2021)..
肝转移是结直肠癌（CRC）相关死亡的主要原因。在这里，我们描述了一种优化的方案，以建立更具临床相关性的 CRC 转移小鼠模型。我们详细介绍了皮下移植表达荧光素酶的 CRC 细胞和随后原位移植皮下肿瘤组织的步骤。这种小鼠模型允许 CRC 细胞在肠道内形成肿瘤，并转移到肝脏，从而提供了评估 CRC 肝转移的体内方法。有关该方案使用和实施的完整详细信息，请参阅 Zhang 等人。（2021 年）。
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