从人骨骼肌中纯化和保存卫星细胞。

Purification and preservation of satellite cells from human skeletal muscle.

机构信息

Program in Craniofacial Biology and Department of Orofacial Sciences, University of California, San Francisco, San Francisco, CA, USA.

Division of Plastic and Reconstructive Surgery, Department of Surgery, University of California, San Francisco, San Francisco, CA, USA.

出版信息

STAR Protoc. 2021 Jan 29;2(1):100302. doi: 10.1016/j.xpro.2021.100302. eCollection 2021 Mar 19.

DOI:10.1016/j.xpro.2021.100302

PMID:33554137

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7851841/

Abstract

Regeneration and repair of skeletal muscle is driven by tissue-specific progenitor cells called satellite cells, which occupy a minority of the cells in the muscle. This protocol provides researchers with techniques to efficiently isolate and purify functional satellite cells from human muscle tissue. The proven techniques described here enable the preparation of purified and minimally altered satellite cells for and experimentation and for potential clinical applications. For complete details on the use and execution of this protocol, please refer to Barruet et al. (2020) and Garcia et al. (2018).

摘要

骨骼肌的再生和修复由组织特异性祖细胞（称为卫星细胞）驱动，这些细胞仅占肌肉细胞的一小部分。本方案为研究人员提供了从人体肌肉组织中有效分离和纯化功能卫星细胞的技术。这里描述的经过验证的技术可用于制备纯化且最小程度改变的卫星细胞，用于和实验以及潜在的临床应用。有关使用和执行本方案的完整详细信息，请参阅 Barruet 等人（2020 年）和 Garcia 等人（2018 年）。