Eccles Institute of Neuroscience, John Curtin School of Medical Research, The Australian National University, Acton, Canberra, ACT 2601, Australia.
The Australian Research Council Centre of Excellence for Integrative Brain Function, ACT Node, Caneberra, ACT 2601, Australia.
STAR Protoc. 2021 Feb 2;2(1):100317. doi: 10.1016/j.xpro.2021.100317. eCollection 2021 Mar 19.
Here, we describe a protocol to simultaneously record and label single cortical neurons under local application of a chemical such as a receptor agonist. This protocol provides a useful tool to investigate how the chemical of interest affects the processing of sensory information by cortical neurons. The juxtacellular labeling allows identification of the cell type and morphology of the recorded neurons. We draw examples to show pharmacological modulations in encoding of vibrotactile stimuli in the mouse primary somatosensory cortex. For complete details on the use and execution of this protocol, please refer to Kheradpezhouh et al. (2020).
在这里，我们描述了一种在局部施加化学物质（如受体激动剂）的情况下同时记录和标记单个皮质神经元的方案。该方案为研究感兴趣的化学物质如何影响皮质神经元对感觉信息的处理提供了有用的工具。细胞外标记允许识别记录神经元的细胞类型和形态。我们举例说明了在小鼠初级体感皮层中振动触觉刺激的编码的药理学调制。有关该方案使用和执行的完整详细信息，请参考 Kheradpezhouh 等人。（2020 年）。
