Biochemistry, Cellular and Molecular Biology Graduate program, Central Michigan University, Mount Pleasant, MI 48859, USA.
College of Medicine, Central Michigan University, Mount Pleasant, MI 48859, USA.
STAR Protoc. 2021 Jul 7;2(3):100667. doi: 10.1016/j.xpro.2021.100667. eCollection 2021 Sep 17.
Bioluminescent optogenetics (BL-OG) allows activation of photosensory proteins, such as opsins, by either fiberoptics or by administering a luciferin. BL-OG thus confers both optogenetic and chemogenetic access within the same genetically targeted neuron. This bimodality offers a powerful approach for non-invasive chemogenetic manipulation of neural activity during brain development and adult behaviors with standard optogenetic spatiotemporal precision. We detail protocols for bioluminescent stimulation of neurons in postnatally developing brain and its validation through bioluminescence imaging and electrophysiological recording in mice. For complete information on the use and execution of this protocol, please refer to Medendorp et al. (2021).
生物发光光学遗传学 (BL-OG) 可通过光纤或施用荧光素来激活光敏蛋白，如视蛋白。因此，BL-OG 在同一基因靶向神经元内同时赋予光遗传学和化遗传学的作用。这种双模性为在大脑发育和成年行为期间，通过标准的光遗传学时空精度，对神经活动进行非侵入性的化遗传学操纵提供了一种强大的方法。我们详细介绍了在出生后发育的大脑中对神经元进行生物发光刺激的方案，并通过在小鼠中的生物发光成像和电生理记录对其进行了验证。如需完整了解此方案的使用和执行信息，请参考 Medendorp 等人 (2021)。
