Department of Immunology, School of Medicine, Jiangsu University, Zhenjiang, Jiangsu Province, China.
Department of Clinical Laboratory, Fujian Medical University Union Hospital, Fuzhou, Fujian Province, China.
Biosci Rep. 2021 Feb 26;41(2). doi: 10.1042/BSR20201158.
MicroRNA (miR) acts as a negative regulator of gene expression. Many literatures have suggested that miRs may be involved in the process of cell proliferation, inflammation, oxidative stress, energy metabolism and epithelial-mesenchymal transition. Thus, miRs may be implicated in the occurrence of non-small cell lung cancer (NSCLC). In the current investigation, we included 2249 subjects (1193 NSCLC patients and 1056 controls) and designed a study to identify the relationship of miR-146a rs2910164 C/G, -499a rs3746444 A/G and -196a-2 rs11614913 T/C with the risk of NSCLC. The risk factors (e.g., body mass index (BMI), sex, smoking, drinking and age) was used to adjust the odds ratios (ORs) and 95% confidence intervals (CIs). After conducting a power value assessment, we did not confirm that the miR-single nucleotide polymorphisms (SNPs) genotypic distributions were different in NSCLC cases and controls. However, the association of miR-196a-2 rs11614913 with a decreased risk of NSCLC was identified in the female subgroup (adjusted P=0.005, power = 0.809 for TC vs. TT, and adjusted P=0.004, power = 0.849 for CC/TC vs. TT). In addition, gene-gene interaction analysis showed that rs11614913 TC/3746444 AA and rs11614913 CC/rs3746444 AA could also reduce the susceptibility to NSCLC (rs11614913 TC/rs3746444 AA vs. rs11614913 TT/rs3746444 AA, P=0.001, power = 0.912 and rs11614913 CC/rs3746444 AA vs. rs11614913 TT/rs3746444 AA, P=0.003, power = 0.836). In conclusion, in overall comparisons, we did not confirm that the rs2910164, rs3746444, and rs11614913 SNPs genotypic distributions were different in NSCLC cases and controls. However, this case-control study demonstrates that miR-196a-2 rs11614913 may be a protective factor for the development of NSCLC among female patients.
微小 RNA（miR）作为基因表达的负调控因子。许多文献表明，miRs 可能参与细胞增殖、炎症、氧化应激、能量代谢和上皮-间充质转化的过程。因此，miRs 可能与非小细胞肺癌（NSCLC）的发生有关。在目前的研究中，我们纳入了 2249 名受试者（1193 名 NSCLC 患者和 1056 名对照者），并设计了一项研究来确定 miR-146a rs2910164 C/G、-499a rs3746444 A/G 和 -196a-2 rs11614913 T/C 与 NSCLC 风险之间的关系。使用风险因素（例如体重指数（BMI）、性别、吸烟、饮酒和年龄）来调整比值比（ORs）和 95%置信区间（CIs）。进行功效值评估后，我们并未确认 miR-单核苷酸多态性（SNP）的基因型分布在 NSCLC 病例和对照组之间存在差异。然而，我们发现 miR-196a-2 rs11614913 与女性亚组中 NSCLC 风险降低相关（调整后的 P=0.005，TC 与 TT 相比的功效=0.809，CC/TC 与 TT 相比的调整后 P=0.004，功效=0.849）。此外，基因-基因相互作用分析表明，rs11614913 TC/3746444 AA 和 rs11614913 CC/rs3746444 AA 也可以降低 NSCLC 的易感性（rs11614913 TC/rs3746444 AA 与 rs11614913 TT/rs3746444 AA 相比，P=0.001，功效=0.912，rs11614913 CC/rs3746444 AA 与 rs11614913 TT/rs3746444 AA 相比，P=0.003，功效=0.836）。总之，在总体比较中，我们并未确认 miR-2910164、rs3746444 和 rs11614913 SNP 的基因型分布在 NSCLC 病例和对照组之间存在差异。然而，这项病例对照研究表明，miR-196a-2 rs11614913 可能是女性 NSCLC 患者发病的保护因素。
