Health and Biomedical Innovation, Clinical and Health Sciences, University of South Australia, Adelaide, South Australia, Australia.
Department of Medical Histology and Cell Biology, Faculty of Medicine, Alexandria University, Alexandria, Egypt.
Stem Cells. 2021 Jun;39(6):803-818. doi: 10.1002/stem.3351. Epub 2021 Feb 13.
The interplay between mesenchymal stem cells (MSCs) and immune cells has been studied for MSCs isolated from different tissues. However, the immunomodulatory capacity of urine stem cells (USCs) has not been adequately researched. The present study reports on the effect of USCs on peripheral blood lymphocytes. USCs were isolated and characterized before coculture with resting and with anti-CD3/CD28 bead stimulated lymphocytes. Similarly to bone marrow mesenchymal stem cells (BM-MSCs), USCs inhibited the proliferation of activated T lymphocytes and induced their apoptosis. However, they also induced strong activation, proliferation, and cytokine and antibody production by B lymphocytes. Molecular phenotype and supernatant analysis revealed that USCs secrete a range of cytokines and effector molecules, known to play a central role in B cell biology. These included B cell-activating factor (BAFF), interleukin 6 (IL-6) and CD40L. These findings raise the possibility of an unrecognized active role for kidney stem cells in modulating local immune cells.
间充质干细胞（MSCs）与免疫细胞之间的相互作用已在不同组织来源的 MSCs 中进行了研究。然而，尿干细胞（USCs）的免疫调节能力尚未得到充分研究。本研究报告了 USCs 对外周血淋巴细胞的影响。在与静止和抗 CD3/CD28 珠刺激的淋巴细胞共培养之前，分离并表征了 USCs。与骨髓间充质干细胞（BM-MSCs）一样，USCs 抑制活化 T 淋巴细胞的增殖并诱导其凋亡。然而，它们还强烈激活、增殖，并诱导 B 淋巴细胞产生细胞因子和抗体。分子表型和上清液分析表明，USCs 分泌一系列细胞因子和效应分子，这些分子在 B 细胞生物学中发挥着核心作用。其中包括 B 细胞激活因子（BAFF）、白细胞介素 6（IL-6）和 CD40L。这些发现提出了肾脏干细胞在调节局部免疫细胞方面可能具有未被认识的积极作用的可能性。
