Ishida Y, Taniguchi H, Baba S
Second Department of Internal Medicine, Kobe University School of Medicine, Japan.
Biochem Biophys Res Commun. 1988 Mar 30;151(3):1122-7. doi: 10.1016/s0006-291x(88)80482-0.
Growth of 3T3-L1 cells was inhibited by 10(-10)-10(-7)M of 1 alpha,25-dihydroxy vitamin D3 [1 alpha,25(OH)2D3] in a dose- and time-dependent manner. The potency of 1 alpha,25(OH)2D3 in inducing differentiation was low, since 3T3-L1 cells cultured with 1 alpha,25(OH)2D3 did not become mature adipocyte-like cells but were changed to slightly rounded cells containing small droplet-like substances in the cytoplasm and glycerophosphate dehydrogenase (sn-glycerol-3-phosphate: NAD+2-oxidoreductase, EC 1.1.1.8), the marker enzyme of differentiation to adipocyte, did not increase. These results together with the natural occurrence of this vitamin indicate that 1 alpha,25(OH)2D3 may play an important role in the cell growth and differentiation besides such known action as intestinal calcium transport and bone mineral mobilization.
1α,25 - 二羟基维生素D3 [1α,25(OH)2D3]在10(-10)-10(-7)M浓度范围内，以剂量和时间依赖性方式抑制3T3 - L1细胞的生长。1α,25(OH)2D3诱导分化的能力较低，因为用1α,25(OH)2D3培养的3T3 - L1细胞未变成成熟的脂肪细胞样细胞，而是变成了细胞质中含有小滴状物质的稍圆细胞，且脂肪细胞分化的标志物酶甘油磷酸脱氢酶（sn - 甘油 - 3 - 磷酸：NAD + 2 - 氧化还原酶，EC 1.1.1.8）未增加。这些结果连同该维生素的天然存在情况表明，1α,25(OH)2D3除了具有如肠道钙转运和骨矿物质动员等已知作用外，可能在细胞生长和分化中发挥重要作用。
