Damante G, Cox F, Rapoport B
Department of Medicine, Veterans' Administration Medical Center, San Francisco, California 94121.
Biochem Biophys Res Commun. 1988 Mar 30;151(3):1194-9. doi: 10.1016/s0006-291x(88)80492-3.
We studied the effect of IGF-1 on c-fos mRNA expression in FRTL5 rat thyroid cells. IFG-1, or calf serum, transiently increased c-fos mRNA levels in quiescent FRTL5 cells, with the latter being more potent. This increase was prevented by actinomycin D. FRTL5 cells were stably transfected with the c-fos promoter linked upstream to the reporter gene chloramphenicol acetyl transferase (CAT). Calf serum and IGF-1 increased c-fos promoter activity in a prolonged manner, and to an equal degree. Our data indicate that IGF-1, as well as additional factor(s) in serum, increase c-fos mRNA levels, at least in part, by activation of the c-fos promoter.
我们研究了胰岛素样生长因子-1（IGF-1）对FRTL5大鼠甲状腺细胞中c-fos信使核糖核酸（mRNA）表达的影响。IGF-1或小牛血清可使静止的FRTL5细胞中的c-fos mRNA水平短暂升高，其中小牛血清的作用更强。放线菌素D可阻止这种升高。将与报告基因氯霉素乙酰转移酶（CAT）上游相连的c-fos启动子稳定转染至FRTL5细胞。小牛血清和IGF-1可长期且同等程度地增加c-fos启动子活性。我们的数据表明，IGF-1以及血清中的其他因子至少部分通过激活c-fos启动子来增加c-fos mRNA水平。
