Damante G, Rapoport B
Department of Medicine, Veterans' Administration Medical Center, San Francisco, CA 94121.
Mol Cell Endocrinol. 1988 Aug;58(2-3):279-82. doi: 10.1016/0303-7207(88)90165-7.
C-fos promoter activity was examined in FRTL5 rat thyroid cells transiently transfected with a chimeric gene containing the c-fos promoter region linked upstream of the receptor gene chloroamphenicol acetyl transferase (CAT). Thyroid-stimulating hormone (TSH) stimulation of transfected cells increased CAT activity 2- to 3-fold. TSH did not stimulate CAT activity driven by the Rous sarcoma virus (RSV) promoter. Both forskolin and 8-Br-cyclic AMP also stimulated CAT activity, and were not additive with TSH stimulation. The kinetics of c-fos-driven CAT activity in response to TSH and cyclic AMP inducers were similar, and stimulation was reversible. These data therefore provide the first evidence that TSH and cyclic AMP stimulate the activity of the c-fos promoter in FRTL5 cells.
在瞬时转染了一个嵌合基因的FRTL5大鼠甲状腺细胞中检测了C-fos启动子活性，该嵌合基因包含与氯霉素乙酰转移酶（CAT）受体基因上游相连的C-fos启动子区域。转染细胞经促甲状腺激素（TSH）刺激后，CAT活性增加了2至3倍。TSH不会刺激由劳氏肉瘤病毒（RSV）启动子驱动的CAT活性。福斯可林和8-溴环磷酸腺苷（8-Br-cyclic AMP）也能刺激CAT活性，且与TSH刺激无叠加效应。响应TSH和环磷酸腺苷诱导剂，由c-fos驱动的CAT活性的动力学相似，且刺激是可逆的。因此，这些数据首次证明TSH和环磷酸腺苷可刺激FRTL5细胞中c-fos启动子的活性。
