Duriatti A, Schuber F
Laboratoire de Chimie Enzymatique, CNRS UA 1182, Strasbourg, France.
Biochem Biophys Res Commun. 1988 Mar 30;151(3):1378-85. doi: 10.1016/s0006-291x(88)80515-1.
2,3-Oxidosqualene-lanosterol cyclase is an intrinsic microsomal protein which can be solubilized by ionic (deoxycholate) and nonionic (emulphogene) detergents with good yields. The hog-liver microsomal cyclase was purified approximately 140-fold by chromatography on DEAE-cellulose and hydroxylapatite. The partially purified enzyme was inactivated by N-ethylmaleimide, following pseudo-first order kinetics, indicating that a cysteine residue is essential for activity.
2,3-氧化角鲨烯-羊毛甾醇环化酶是一种内在微粒体蛋白，可通过离子型（脱氧胆酸盐）和非离子型（乳化剂）去污剂以高产率溶解。猪肝微粒体环化酶通过在DEAE-纤维素和羟基磷灰石上进行层析，纯化了约140倍。部分纯化的酶被N-乙基马来酰亚胺灭活，遵循假一级动力学，表明半胱氨酸残基对活性至关重要。
