Martin M, Krichbaum M, Kaever V, Goppelt-Strübe M, Resch K
Department of Pharmacology and Toxicology, Medical School, Hannover, Federal Republic of Germany.
Biochem Pharmacol. 1988 Mar 15;37(6):1083-8. doi: 10.1016/0006-2952(88)90514-x.
Rat glomerular mesangial cells were isolated and grown in culture for a prolonged period of time. The proliferation of these cells was suppressed by the immunosuppressivum Cyclosporin A (CSA) up to 75%, as measured by the incorporation of radiolabeled thymidine. This was dependent on the concentration of CSA used, being in the range of 125-2000 ng/ml. This effect was specific for CSA and other immunosuppressive derivatives thereof, while a non-immunosuppressive cyclosporin was ineffective. Neither the density of the cultures, nor the time of application had any influence on the susceptibility of the cells to CSA. The suppression of MC proliferation was proliferation was reversible after removal of CSA. These studies demonstrate a suppressive effect of Cyclosporin A on the proliferation of non-lymphocytic cells, the glomerular mesangial cells. This observation may help to explain the beneficial results reported with CSA in the treatment of some kidney diseases.
大鼠肾小球系膜细胞被分离出来并在培养中长时间生长。通过放射性标记的胸腺嘧啶核苷掺入法测定，这些细胞的增殖被免疫抑制剂环孢素A（CSA）抑制高达75%。这取决于所使用的CSA浓度，范围为125 - 2000 ng/ml。这种作用对CSA及其其他免疫抑制衍生物具有特异性，而一种非免疫抑制性环孢菌素则无效。培养物的密度和应用时间对细胞对CSA的敏感性均无任何影响。去除CSA后，MC增殖的抑制是可逆的。这些研究证明了环孢素A对非淋巴细胞（肾小球系膜细胞）增殖的抑制作用。这一观察结果可能有助于解释在某些肾脏疾病治疗中使用CSA所报告的有益效果。
