Infectious Diseases Research Center, AJA University of Medical Sciences, Tehran, Iran.
Department of Microbiology, Iranian Reference Health Laboratory Research Center, Ministry of Health and Medical Education, Tehran, Iran.
Microb Pathog. 2021 Apr;153:104789. doi: 10.1016/j.micpath.2021.104789. Epub 2021 Feb 6.
Resistance-Nodulation-Division (RND) efflux pumps are responsible for multidrug resistance in Pseudomonas aeruginosa. The present study aimed to evaluate the overexpression of RND efflux pumps and its role in the antibiotic resistance of P. aeruginosa clinical isolates. A number of 122 isolates were obtained from three military hospitals in Tehran, Iran. In order to determine the antibiotic resistance, the isolates were identified and assessed by the disk diffusion and agar dilution methods. This study investigated the gene expression of four multi-drug efflux pump systems (MexAB-OprM, MexCD-OprJ, MexEF-OprN and MexXY (-OprA)) and its correlation with antibiotic resistance. The isolates indicated that the highest resistance rate was against ticarcillin (80%), followed by ciprofloxacin (74%) and meropenem (71%). Most of them expressed mexB (69%), mexC (28.7%), mexE (43.4%), and mexY (74.6%), suggesting that mexB and mexY were highly expressed in the studied strains. The overexpression of mexB and mexY was significantly more prevalent in the ICU wards (p = 0.033). Furthermore, there was a significant correlation between the expression of RND-type efflux pumps and the resistance to most anti-pseudomonal antibiotics.
耐药-结节-分裂（RND）外排泵负责铜绿假单胞菌的多药耐药性。本研究旨在评估 RND 外排泵的过度表达及其在铜绿假单胞菌临床分离株抗生素耐药性中的作用。从伊朗德黑兰的三家军事医院获得了 122 株分离株。为了确定抗生素耐药性，通过纸片扩散和琼脂稀释法对分离株进行了鉴定和评估。本研究调查了四个多药外排泵系统（MexAB-OprM、MexCD-OprJ、MexEF-OprN 和 MexXY (-OprA)）的基因表达及其与抗生素耐药性的相关性。分离株表明，对替卡西林（80%）的耐药率最高，其次是环丙沙星（74%）和美罗培南（71%）。它们中的大多数表达了 mexB（69%）、mexC（28.7%）、mexE（43.4%）和 mexY（74.6%），这表明在研究的菌株中 mexB 和 mexY 表达较高。在 ICU 病房中，mexB 和 mexY 的过度表达更为普遍（p=0.033）。此外，RND 型外排泵的表达与大多数抗假单胞菌抗生素的耐药性之间存在显著相关性。
