Department of Analytical Chemistry, University of Córdoba, Faculty of Sciences, International Agri-food Campus of Excellence (ceiA3), Marie Curie Annex Building, Campus de Rabanales, 14071 Córdoba, Spain.
Department of Analytical Chemistry, University of Córdoba, Faculty of Sciences, International Agri-food Campus of Excellence (ceiA3), Marie Curie Annex Building, Campus de Rabanales, 14071 Córdoba, Spain.
J Chromatogr A. 2021 Mar 15;1640:461937. doi: 10.1016/j.chroma.2021.461937. Epub 2021 Jan 29.
The potential of headspace-gas chromatography-ion mobility spectrometry (HS-GC-IMS) to perform non-targeted qualitative analysis of complex samples has led to an unprecedented increase in its popularity in recent years. The operating principle of IMS makes quality control essential to ensure adequate results. Besides this, the suitability of GC-IMS is determined by multiple phenomena that take place before and during IMS detection. The present work discusses a novel GC-IMS quality control protocol for both beginners and experienced users. Likewise, it describes factors that must be taken into account in order to develop a robust GC-IMS qualitative analysis method and, if needed, to achieve the identification of VOCs present in real samples. The developed quality control protocol was successfully employed in our laboratory for the routine analysis of >500 real samples (olive oil and Iberian ham) for 6 months, thus it is recommended for the analysis of a great number of complex samples. Furthermore, the behaviour of the ions produced in the ionisation chamber and the possible reactions between them in GC-IMS qualitative analysis were assessed.
顶空-气相色谱-离子迁移谱（HS-GC-IMS）在对复杂样品进行非靶向定性分析方面具有巨大潜力，近年来其应用得到了前所未有的普及。IMS 的工作原理使得质量控制成为确保获得可靠结果的必要条件。此外，GC-IMS 的适用性取决于 IMS 检测前后发生的多种现象。本工作为初学者和有经验的用户讨论了一种新型的 GC-IMS 质量控制方案。同时，还描述了为开发稳健的 GC-IMS 定性分析方法并在必要时实现对实际样品中 VOCs 的鉴定必须考虑的因素。该质量控制方案在本实验室成功用于 6 个月内对 >500 个实际样品（橄榄油和伊比利亚火腿）的常规分析，因此建议用于分析大量复杂样品。此外，评估了在 IMS 定性分析中在离子化室内产生的离子的行为以及它们之间可能发生的反应。
