Johannes Gutenberg-Universität Mainz, Department of Chemistry, Duesbergweg 10-14, 55128 Mainz, Germany.
Fraunhofer Institute for Microengineering and Microsystems IMM, Carl-Zeiss-Str. 18-20, 55129 Mainz, Germany.
J Chromatogr A. 2021 Mar 15;1640:461941. doi: 10.1016/j.chroma.2021.461941. Epub 2021 Jan 27.
The steadily rising interest in the investigation of interactions between nanomaterials and biological media has also led to an increasing interest in asymmetrical flow field-flow fractionation (AF-FFF). The biggest strength of AF-FFF is the possibility to alter the flow profiles to suit a specific separation problem. In this paper, the influence of an oscillating main flow on the separation efficiency of AF-FFF is investigated. Such oscillations can e.g. be caused by the main pump To investigate the influence of such flow conditions on the separation efficiency in AF-FFF systematically, different oscillation profiles were applied and their influence on the elution profile and the retention times was observed. It could be shown, that the separation mechanism is extremely robust and a fractionation is still possible even under unfavorable conditions.
人们对纳米材料与生物介质相互作用的研究兴趣日益浓厚，这也促使人们对不对称流场流分离（AF-FFF）越来越感兴趣。AF-FFF 的最大优势在于能够改变流型以适应特定的分离问题。本文研究了在 AF-FFF 中，主流动的振荡对分离效率的影响。例如，这种振荡可能是由主泵引起的。为了系统地研究这种流动条件对 AF-FFF 中分离效率的影响，本文应用了不同的振荡轮廓，并观察了它们对洗脱轮廓和保留时间的影响。结果表明，分离机制非常稳健，即使在不利的条件下，仍可实现分离。
