Okuno F, Ishii H, Kashiwazaki K, Takagi S, Shigeta Y, Arai M, Takagi T, Ebihara Y, Tsuchiya M
Department of Internal Medicine, School of Medicine, University of Occupational and Environmental Health, Kitakyushu, Japan.
Alcohol. 1988 Jan-Feb;5(1):49-53. doi: 10.1016/0741-8329(88)90043-2.
In order to clarify the origin and the mechanism of increased serum activity of glutamic oxalacetic transaminase (GOT) in chronic alcoholics, clinical and experimental investigations were carried out. Mitochondrial (m-GOT) and cytosolic GOT (c-GOT) isoenzymes were separated chromatographically by using a mini-column packed with Sephadex A50. Sixty percent of 63 alcoholics had elevated serum GOT. The m-GOT activity in alcoholics with total serum GOT activity of over 50 Karmen Units was 17.2 +/- 1.6 K.U. and the m-GOT/GOT ratio was the highest when compared to those in non-alcoholic liver diseases. In in vitro study, six hours of incubation of isolated hepatocytes from rats fed ethanol chronically resulted in an increased leakage of m-GOT into the incubation medium and also showed a tendency of a higher m-GOT/GOT ratio than that from control rats. The m-GOT activity thus released into the medium showed a highly significant inverse correlation with the viability of hepatocytes. These data suggest that m-GOT substantially contributes to an increased serum GOT often observed in chronic alcoholics.
为了阐明慢性酒精中毒患者血清谷氨酸草酰乙酸转氨酶（GOT）活性升高的起源和机制，进行了临床和实验研究。采用装有Sephadex A50的微型柱，通过色谱法分离线粒体（m-GOT）和胞质GOT（c-GOT）同工酶。63名酗酒者中有60%血清GOT升高。血清总GOT活性超过50卡门单位的酗酒者，其m-GOT活性为17.2±1.6 K.U.，与非酒精性肝病患者相比，m-GOT/GOT比值最高。在体外研究中，对长期喂食乙醇的大鼠分离的肝细胞进行6小时孵育，导致m-GOT向孵育培养基中的泄漏增加，并且m-GOT/GOT比值也有高于对照大鼠的趋势。释放到培养基中的m-GOT活性与肝细胞活力呈高度显著的负相关。这些数据表明，m-GOT在很大程度上导致了慢性酒精中毒患者经常出现的血清GOT升高。
