Animal Technology and Research Center (CITA-IVIA), Polígono La Esperanza nº 100, 12400 Segorbe, Castellón, Spain.
Institute of Science and Animal Technology (ICTA), Universitat Politècnica de València, 46022 Valencia, Spain.
Anim Reprod Sci. 2021 Mar;226:106714. doi: 10.1016/j.anireprosci.2021.106714. Epub 2021 Feb 2.
Effects were analysed of dextran supplementation to MeSO and acetamide rabbit semen freezing extenders on quality characteristics of rabbit spermatozoa and reproductive performance. The final concentration of cryoprotectants in pooled semen samples was 12.4 % MeSO for the A extenders, 10.7 % MeSO and 2.9 % acetamide for the D extenders and 8.9 % MeSO and 2.9 % acetamide in F extenders, with a supplementation of 1.7 % sucrose in all cases. There was not inclusion of dextran in the A0, D0, F0; while 5 % dextran was included in A5, D5, F5 and 10 % dextran in A10, D10 and F10 extenders. Sperm motility and viability rates were similar with use of the different extenders. Acrosome integrity after the freeze-thawing processes, however, was markedly greater when there was dextran supplementation of D and F extenders. Prolificacy was affected by extender composition. When there was artificial insemination (AI) using semen cryopreserved in the A extenders, number of kits born was similar to when there was AI with fresh semen when there was inclusion of 5% dextran for cryopreservation, while there was no effect on prolificacy when there was cryopreservation of semen using the D and F extenders. In conclusion, dextran supplementation of extenders containing MeSO and acetamide resulted in greater acrosome integrity. Furthermore, when there was AI using sperm preserved in cryo-diluents containing an intermediate concentration of MeSO, combined with inclusion of 5 % dextran, there was a marked beneficial effect on rabbit doe reproductive performance.
葡聚糖对 MeSO 和乙酰胺兔精液冷冻稀释液的补充作用对精子质量特征和繁殖性能的影响。在 pooled 精液样本中，冷冻保护剂的最终浓度为 A 稀释液中的 12.4% MeSO、D 稀释液中的 10.7% MeSO 和 2.9% 乙酰胺以及 F 稀释液中的 8.9% MeSO 和 2.9% 乙酰胺，所有情况下均添加 1.7% 蔗糖。A0、D0 和 F0 中不包含葡聚糖；A5、D5 和 F5 中包含 5%葡聚糖，A10、D10 和 F10 中包含 10%葡聚糖。使用不同的稀释液，精子活力和活力率相似。然而，在冷冻解冻过程后，当 D 和 F 稀释液中添加葡聚糖时，顶体完整性明显增加。繁殖力受稀释液组成的影响。当使用 A 稀释液中冷冻保存的精液进行人工授精（AI）时，包含 5%葡聚糖进行冷冻保存时，出生的套件数量与新鲜精液进行 AI 时相似，而当使用 D 和 F 稀释液冷冻保存精液时，对繁殖力没有影响。总之，在含有 MeSO 和乙酰胺的稀释液中添加葡聚糖可提高顶体完整性。此外，当使用含有中等浓度 MeSO 的冷冻稀释液保存的精子进行 AI 时，结合添加 5%葡聚糖，对母兔的繁殖性能有明显的有益影响。
