Jackson L C, Oseguera M, Medrano S, Kim Y L
Department of Anthropology, University of Florida, Gainesville 32611.
Biochem Med Metab Biol. 1988 Feb;39(1):64-8. doi: 10.1016/0885-4505(88)90059-x.
Carbamylation of the hemoglobin in sickle cell anemia has been demonstrated to improve the status of this hemoglobinopathy. Using an animal model of West African human patterns of chronic sublethal dietary cyanide ingestion, 12 miniature swine consuming either 0, 0.4, 0.7, or 1.2 mg of cyanide/kg body weight/day were studied for 24 weeks to determine if this dietary regime could produce irreversible carbamylated hemoglobin. Throughout the study, the hematological status of all animals remained similar; however, the levels of carbamylated hemoglobin as measured by nanomoles of valine hydantoin varied proportionally to dietary sublethal cyanide intakes, indicating that these natural dietary levels could effect an important and presumably permanent modification of the hemoglobin's beta chain. Serum thiocyanate levels were also positively correlated with cyanide ingestion loads (r = 0.83, P less than 0.01). The implications of these findings in swine are important for the millions of humans with hemoglobin S who regularly consume similar levels of dietary cyanide and for our assessments of the biochemical and medical status of hemoglobin S under natural conditions.
已证明镰状细胞贫血中血红蛋白的氨甲酰化可改善这种血红蛋白病的状况。利用西非人类慢性亚致死性膳食氰化物摄入模式的动物模型，对12只每天分别摄入0、0.4、0.7或1.2毫克氰化物/千克体重的小型猪进行了24周的研究，以确定这种饮食方案是否会产生不可逆的氨甲酰化血红蛋白。在整个研究过程中，所有动物的血液学状况保持相似；然而，通过缬氨酸乙内酰脲的纳摩尔数测量的氨甲酰化血红蛋白水平与膳食亚致死性氰化物摄入量成比例变化，表明这些天然膳食水平可对血红蛋白的β链产生重要且可能是永久性的修饰。血清硫氰酸盐水平也与氰化物摄入负荷呈正相关（r = 0.83，P < 0.01）。这些在猪身上的发现对于数百万经常摄入类似水平膳食氰化物的血红蛋白S型人类，以及我们对自然条件下血红蛋白S的生化和医学状况的评估具有重要意义。
