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MAR1通过SIRT1/PGC-1α/PPAR-γ途径抑制脂多糖诱导的RAW 264.7巨噬细胞和人原代外周血单个核细胞中的炎症反应。

MAR1 suppresses inflammatory response in LPS-induced RAW 264.7 macrophages and human primary peripheral blood mononuclear cells via the SIRT1/PGC-1α/PPAR-γ pathway.

作者信息

Wang Wei, Xu Rong-Li, He Ping, Chen Rui

机构信息

Department of Emergency, Hainan General Hospital, Hainan Affiliated Hospital of Hainan Medical University, No.19, Xiuhua Road, 570311, Haikou, Hainan Province, People's Republic of China.

Department of Cardiology, Hainan General Hospital, Hainan Affiliated Hospital of Hainan Medical University, No.19, Xiuhua Road, 570311, Haikou, Hainan Province, People's Republic of China.

出版信息

J Inflamm (Lond). 2021 Feb 8;18(1):8. doi: 10.1186/s12950-021-00271-x.

DOI:10.1186/s12950-021-00271-x
PMID:33557833
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7869219/
Abstract

BACKGROUND

Sepsis is a complex syndrome characterized by a dysregulated inflammatory response to systemic infection and leads to shock, multiple organ failure and death especially if not recognized early and treated promptly. Previous studies have suggested Maresin 1 (MAR1) can alleviate systemic inflammation in sepsis, but its mechanism has not been clarified.

METHODS

RAW 264.7 cells and human primary peripheral blood mononuclear cells (hPBMCs) were pretreated with LPS and MAR1. The mRNA expression and supernatant levels of pro-inflammatory cytokines, tumor necrosis factor (TNF-α), interleukin (IL)-1β and IL-6 were evaluated by RT-qPCR and ELISA, respectively. The expression levels of Sirtuin 1 (SIRT1), peroxisome proliferator-activated receptor γ coactivator-1α (PGC-1α), and Peroxisome proliferator-activated receptor gamma (PPAR-γ) were determined by RT-qPCR and Western blot analysis, respectively.

RESULTS

Our results show that LPS-induced inflammation increased the expression and secretion of proinflammatory cytokines TNF-α, IL-1β and IL-6 and induced suppression of SIRT1, PGC-1α, and PPAR-γ expression, which could be reversed by MAR1. And the effect of MAR1 was eliminated by repression of SIRT1/PPAR-γ and enhanced by PGC-1α overexpression.

CONCLUSIONS

MAR1 suppressed inflammatory response in LPS-induced RAW 264.7 macrophages and hPBMCs via the SIRT1/PGC-1α/PPAR-γ pathway.

摘要

背景

脓毒症是一种复杂的综合征，其特征为对全身感染的炎症反应失调，若不及早识别和及时治疗，会导致休克、多器官功能衰竭甚至死亡。既往研究提示maresin 1（MAR1）可减轻脓毒症中的全身炎症，但具体机制尚未阐明。

方法

用脂多糖（LPS）和MAR1预处理RAW 264.7细胞和人原代外周血单核细胞（hPBMCs）。分别通过逆转录定量聚合酶链反应（RT-qPCR）和酶联免疫吸附测定（ELISA）评估促炎细胞因子肿瘤坏死因子（TNF-α）、白细胞介素（IL）-1β和IL-6的mRNA表达及上清液水平。分别通过RT-qPCR和蛋白质免疫印迹分析测定沉默调节蛋白1（SIRT1）、过氧化物酶体增殖物激活受体γ共激活因子-1α（PGC-1α）和过氧化物酶体增殖物激活受体γ（PPAR-γ）的表达水平。

结果

我们的结果显示，LPS诱导的炎症增加了促炎细胞因子TNF-α、IL-1β和IL-6的表达与分泌，并诱导了SIRT1、PGC-1α和PPAR-γ表达的抑制，而MAR1可使其逆转。并且，抑制SIRT1/PPAR-γ可消除MAR1的作用，而过表达PGC-1α可增强其作用。

结论

MAR1通过SIRT1/PGC-1α/PPAR-γ途径抑制LPS诱导的RAW 264.7巨噬细胞和hPBMCs中的炎症反应。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/803a/7869219/92676533992a/12950_2021_271_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/803a/7869219/d0637eb71f88/12950_2021_271_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/803a/7869219/0b18f3452ad1/12950_2021_271_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/803a/7869219/d9d9eade8c5d/12950_2021_271_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/803a/7869219/92676533992a/12950_2021_271_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/803a/7869219/d0637eb71f88/12950_2021_271_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/803a/7869219/0b18f3452ad1/12950_2021_271_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/803a/7869219/d9d9eade8c5d/12950_2021_271_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/803a/7869219/92676533992a/12950_2021_271_Fig5_HTML.jpg

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1
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J Pharm Pharmacol. 2019 Jul;71(7):1142-1151. doi: 10.1111/jphp.13087. Epub 2019 Mar 13.
2
Western diet regulates immune status and the response to LPS-driven sepsis independent of diet-associated microbiome.西方饮食通过调节免疫状态和对脂多糖（LPS）驱动的败血症的反应，独立于饮食相关微生物组。
Proc Natl Acad Sci U S A. 2019 Feb 26;116(9):3688-3694. doi: 10.1073/pnas.1814273116. Epub 2019 Feb 11.
3
Sonchus oleraceus Linn protects against LPS-induced sepsis and inhibits inflammatory responses in RAW264.7 cells.
慢性炎症的消退、表观遗传紊乱的恢复和微生态失调的纠正作为特应性皮炎治疗的辅助方法。
Cells. 2024 Nov 18;13(22):1899. doi: 10.3390/cells13221899.
4
Herbacetin ameliorates lipopolysaccharide-elicited inflammatory response by suppressing NLRP-3/AIM-2 inflammasome activation, PI3K/Akt/MAPKs/NF-κB redox inflammatory signalling, modulating autophagy and macrophage polarization imbalance.芹黄素通过抑制 NLRP-3/AIM-2 炎性小体激活、PI3K/Akt/MAPKs/NF-κB 氧化还原炎症信号通路、调节自噬和巨噬细胞极化失衡，改善脂多糖诱导的炎症反应。
Mol Biol Rep. 2024 Nov 16;51(1):1159. doi: 10.1007/s11033-024-10068-9.
5
Facile Preparation of Multifunctional Hydrogels with Sustained Resveratrol Release Ability for Bone Tissue Regeneration.具有持续白藜芦醇释放能力的多功能水凝胶用于骨组织再生的简便制备方法。
Gels. 2024 Jun 28;10(7):429. doi: 10.3390/gels10070429.
6
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Cell Commun Signal. 2023 May 4;21(1):98. doi: 10.1186/s12964-023-01054-y.
10
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Nutrients. 2023 Mar 27;15(7):1626. doi: 10.3390/nu15071626.
菊苣可预防脂多糖诱导的败血症，并抑制 RAW264.7 细胞的炎症反应。
J Ethnopharmacol. 2019 May 23;236:63-69. doi: 10.1016/j.jep.2019.02.039. Epub 2019 Feb 23.
4
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5
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6
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8
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9
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Lab Invest. 2018 Jun;98(6):715-733. doi: 10.1038/s41374-018-0031-x. Epub 2018 Feb 21.
10
Heme oxygenase-1 induction by rosiglitazone via PKCα/AMPKα/p38 MAPKα/SIRT1/PPARγ pathway suppresses lipopolysaccharide-mediated pulmonary inflammation.罗格列酮通过 PKCα/AMPKα/p38MAPKα/SIRT1/PPARγ 通路诱导血红素加氧酶-1 抑制脂多糖介导的肺部炎症。
Biochem Pharmacol. 2018 Feb;148:222-237. doi: 10.1016/j.bcp.2017.12.024. Epub 2018 Jan 5.