Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang, 611130, China.
Department of Chemistry, College of Life and Basic Science, Sichuan Agricultural University, Wenjiang, 611130, China.
Parasit Vectors. 2021 Feb 8;14(1):103. doi: 10.1186/s13071-021-04596-7.
Cystic echinococcosis is a parasitic zoonotic disease, which poses a threat to public health and animal husbandry, and causes significant economic losses. Annexins are a family of phospholipid-binding proteins with calcium ion-binding activity, which have many functions.
Two annexin protein family genes [Echinococcus granulosus annexin B3 (EgAnxB3) and EgAnxB38] were cloned and molecularly characterized using bioinformatic analysis. The immunoreactivity of recombinant EgAnxB3 (rEgAnxB3) and rEgAnxB38 was investigated using western blotting. The distribution of EgAnxB3 and EgAnxB38 in protoscoleces (PSCs), the germinal layer, 18-day strobilated worms and 45-day adult worms was analyzed by immunofluorescence localization, and their secretory characteristics were analyzed preliminarily; in addition, quantitative real-time reverse transcription polymerase chain reaction was used to analyze their transcript levels in PSCs and 28-day strobilated worms stages. The phospholipid-binding activities of rEgAnxB3 and rEgAnxB38 were also analyzed.
EgAnxB3 and EgAnxB38 are conserved and contain calcium-binding sites. Both rEgAnxB3 and rEgAnxB38 could be specifically recognized by the serum samples from E. granulosus-infected sheep, indicating that they had strong immunoreactivity. EgAnxB3 and EgAnxB38 were distributed in all stages of E. granulosus, and their transcript levels were high in the 28-day strobilated worms. They were found in liver tissues near the cysts. In addition, rEgAnxB3 has Ca-dependent phospholipid-binding properties.
EgAnxB3 and EgAnxB38 contain calcium-binding sites, and rEgAnxB3 has Ca-dependent phospholipid-binding properties. EgAnxB3 and EgAnxB38 were transcribed in PSCs and 28-day strobilated worms. They were expressed in all stages of E. granulosus, and distributed in the liver tissues near the hydatid cyst, indicating that they are secreted proteins that play a crucial role in the development of E. granulosus.
包虫病是一种寄生虫性人畜共患疾病，对公共卫生和畜牧业构成威胁，并造成重大经济损失。膜联蛋白是一类具有钙离子结合活性的磷脂结合蛋白，具有多种功能。
利用生物信息学分析，克隆并分子鉴定了两个膜联蛋白家族基因[细粒棘球蚴膜联蛋白 B3（EgAnxB3）和 EgAnxB38]。采用 Western blot 法检测重组 EgAnxB3（rEgAnxB3）和 rEgAnxB38 的免疫反应性。通过免疫荧光定位分析，研究了 EgAnxB3 和 EgAnxB38 在原头蚴（PSCs）、生发层、18 日龄具纤毛幼虫和 45 日龄成虫中的分布，并初步分析了它们的分泌特性；此外，还采用实时定量逆转录聚合酶链反应分析了它们在 PSCs 和 28 日龄具纤毛幼虫阶段的转录水平。还分析了 rEgAnxB3 和 rEgAnxB38 的磷脂结合活性。
EgAnxB3 和 EgAnxB38 具有保守性且含有钙结合位点。rEgAnxB3 和 rEgAnxB38 均能被感染细粒棘球蚴的绵羊血清样本特异性识别，表明它们具有很强的免疫反应性。EgAnxB3 和 EgAnxB38 分布于细粒棘球蚴的各个阶段，其转录水平在 28 日龄具纤毛幼虫中较高。它们在靠近包虫囊肿的肝脏组织中被发现。此外，rEgAnxB3 具有 Ca 依赖性磷脂结合特性。
EgAnxB3 和 EgAnxB38 含有钙结合位点，rEgAnxB3 具有 Ca 依赖性磷脂结合特性。EgAnxB3 和 EgAnxB38 在 PSCs 和 28 日龄具纤毛幼虫中转录。它们在细粒棘球蚴的各个阶段表达，并分布在靠近肝包虫囊肿的肝脏组织中，表明它们是分泌蛋白，在细粒棘球蚴的发育中起关键作用。
