分离的小牛肺动脉内皮细胞的渗透通透性。

The osmotic permeability of isolated calf pulmonary artery endothelial cells.

作者信息

Garrick R A, DiRisio D J, Giannuzzi R, Cua W O, Ryan U S, Chinard F P

机构信息

Division of Science and Mathematics, Fordham University, College at Lincoln Center, New York, NY.

出版信息

Biochim Biophys Acta. 1988 Apr 7;939(2):343-8. doi: 10.1016/0005-2736(88)90079-x.

DOI:10.1016/0005-2736(88)90079-x

PMID:3355822

Abstract

Osmotic permeability coefficients, PF, for water in isolated calf pulmonary artery endothelial cells determined over the temperature range 41 to 20 degrees C are 311.10(-5) cm.s-1 at 37 degrees C and 159.10(-5) cm.s-1 at 20 degrees C. The value at 37 degrees C is close to that reported earlier for the diffusional permeability coefficient, PD. The PF/PD ratio is 1.0 at 37 degrees C. The PF values are within the range of values extrapolated for filtration permeability in pulmonary endothelium. The temperature dependence expressed as the activation energy is 7.2 kcal.mol-1. The product of hydraulic conductivity, Lp (or PF) and of viscosity changes in water is not constant from 37 to 20 degrees C. These results can be interpreted to indicate a similar pathway for water whether under diffusional or osmotic gradients.

摘要

在41至20摄氏度温度范围内测定的分离小牛肺动脉内皮细胞中水的渗透渗透系数PF，在37摄氏度时为311×10⁻⁵厘米·秒⁻¹，在20摄氏度时为159×10⁻⁵厘米·秒⁻¹。37摄氏度时的值接近先前报道的扩散渗透系数PD的值。37摄氏度时PF/PD比值为1.0。PF值在肺内皮过滤渗透率外推值范围内。以活化能表示的温度依赖性为7.2千卡·摩尔⁻¹。从37摄氏度到20摄氏度，水力传导率Lp（或PF）与水的粘度变化的乘积不是恒定的。这些结果可以解释为表明水在扩散或渗透梯度下具有相似的途径。