Sloop C H, Dory L, Krause B R, Castle C, Roheim P S
Atherosclerosis. 1983 Oct;49(1):9-21. doi: 10.1016/0021-9150(83)90003-5.
Prenodal peripheral lymph was used as a model of interstitial fluid to obtain information on the composition of lipoproteins and apolipoproteins which are in direct contact with peripheral cells. Lipoproteins resembling plasma lipoproteins in size and electrophoretic mobility were present in the prenodal peripheral lymph of control as well as cholesterol-fed dogs. Most of the lipoproteins in control dogs were high density lipoproteins, both in the plasma and in the lymph. Cholesterol feeding resulted in an increased concentration of lipoprotein particles with decreased electrophoretic mobility (beta-VLDL and, in plasma, HDLc) and decreased concentration of HDL, both in plasma and in lymph. Size distribution of lipoproteins was also markedly altered by cholesterol feeding; most of the lipoproteins were present as IDL and VLDL both in plasma and in lymph. Judged by agarose gel chromatography, the size of the lymph HDL as consistently larger than plasma HDL in both groups of dogs. Furthermore, it appears that cholesterol feeding increased the size of an HDL subfraction, partially resolved by agarose chromatography, both in lymph and plasma. All apolipoproteins present in plasma were also present in lymph. Cholesterol feeding resulted in 3-10-fold increases in plasma apo B, E, and A-IV while apo A-I was drastically decreased. These changes were reflected in lymph to different degrees depending on the size of the lipoprotein fraction containing the individual apolipoproteins. Our findings provide direct evidence that the large, cholesterol-rich, 'atherogenic' lipoproteins found in the plasma of cholesterol-fed dogs (beta-VLDL) are also present in the interstitial fluid and presumably interact with peripheral cells. Our studies furthermore suggest modification of plasma HDL by peripheral cells and/or de novo assembly of an HDL subfraction. The utilization of this animal model may thus provide a direct approach to the study of the interaction of lipoproteins with peripheral cells.
结前外周淋巴被用作组织液模型，以获取与外周细胞直接接触的脂蛋白和载脂蛋白组成的信息。在对照犬以及喂食胆固醇的犬的结前外周淋巴中，存在大小和电泳迁移率与血浆脂蛋白相似的脂蛋白。对照犬血浆和淋巴中的大多数脂蛋白都是高密度脂蛋白。喂食胆固醇导致脂蛋白颗粒浓度增加，电泳迁移率降低（β-VLDL，以及血浆中的HDLc），血浆和淋巴中的HDL浓度降低。喂食胆固醇也显著改变了脂蛋白的大小分布；血浆和淋巴中的大多数脂蛋白以中间密度脂蛋白（IDL）和极低密度脂蛋白（VLDL）形式存在。通过琼脂糖凝胶色谱判断，两组犬淋巴HDL的大小始终大于血浆HDL。此外，喂食胆固醇似乎增加了琼脂糖色谱部分分离的HDL亚组分的大小，在淋巴和血浆中均如此。血浆中存在的所有载脂蛋白在淋巴中也存在。喂食胆固醇导致血浆载脂蛋白B、E和A-IV增加3至10倍，而载脂蛋白A-I急剧下降。这些变化在淋巴中根据含有各个载脂蛋白的脂蛋白部分的大小而有不同程度的反映。我们的研究结果提供了直接证据，表明在喂食胆固醇的犬血浆中发现的大的、富含胆固醇的“致动脉粥样硬化”脂蛋白（β-VLDL）也存在于组织液中，并可能与外周细胞相互作用。我们的研究还表明外周细胞对血浆HDL的修饰和/或HDL亚组分的从头组装。因此，利用这种动物模型可能为研究脂蛋白与外周细胞的相互作用提供一种直接方法。
