Institute of Molecular Biology, University of Duisburg-Essen, Hufelandstrasse 55, 45122 Essen, Germany.
Department of Hematology, University Hospital Essen, University of Duisburg-Essen, Hufelandstrasse 55, 45122 Essen, Germany.
STAR Protoc. 2021 Mar 19;2(1):100356. doi: 10.1016/j.xpro.2021.100356. Epub 2021 Feb 3.
This protocol enables the testing of drugs against infection of epithelial cells with SARS-CoV-2 (severe acute respiratory syndrome coronavirus-2), using pseudo-typed replication deficient vesicular stomatitis virus particles (pp-VSV) presenting the SARS-CoV-2 spike protein. After treating human volunteers with amitriptyline, an approved antidepressant and inhibitor of the acid sphingomyelinase, freshly isolated nasal epithelial cells were infected and infection levels were quantified. This protocol offers the possibility to rapidly test the efficacy of potential drugs in the fight against COVID-19. For complete details on the use and execution of this protocol, please refer to Carpinteiro et al. (2020).
本方案使用携带 SARS-CoV-2（严重急性呼吸综合征冠状病毒-2）刺突蛋白的假型复制缺陷型水疱性口炎病毒颗粒（pp-VSV）来检测药物对感染上皮细胞的作用。先用阿米替林（一种已批准的抗抑郁药和酸性鞘磷脂酶抑制剂）处理志愿者，然后用新鲜分离的鼻上皮细胞进行感染，并对感染水平进行定量。本方案提供了快速测试针对 COVID-19 的潜在药物疗效的可能性。有关本方案使用和执行的详细信息，请参考 Carpinteiro 等人（2020 年）。
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