Laboratory of Insect and Vectors, Department of Microbiology and Parasitology, Federal University of Rio Grande do Norte, Av. Sen. Salgado Filho, Lagoa Nova, Natal, RN, Brazil.
Department of Biophysics and Pharmacology, Federal University of Rio Grande do Norte, Av. Sen. Salgado Filho, Lagoa Nova, Natal, RN, Brazil.
J Med Entomol. 2021 May 15;58(3):1056-1063. doi: 10.1093/jme/tjaa284.
The determination of necrophagous fly specie's development time is considered an accurate method for estimating postmortem interval (PMI). However, pesticides and other chemicals can alter the flies' life cycle, inducing errors in PMI estimation. Thus, this work aimed to evaluate the effect of different doses of Terbufos (Organophosphates) on the temporal dispersion pattern and development of immature dipterans associated with decaying rat carcasses. For this, 150 g female Wistar rats received, via gavage, 200 µl of Terbufos (5 or 10 mg/kg) or distilled water (control) and, after 30 min of administration, the animals were euthanized and distributed in suspended traps to decompose under environmental conditions. The dispersing immatures were collected daily, and their development time was monitored until the emergence of adult flies. After data analysis, it was observed that Terbufos altered 1) the temporal pattern of larval dispersion; 2) the composition and structure of the colonizing assemblage (emerged adults); 3) species' development time, accelerating or delaying their cycle, depending on the dose used; and 4) the califorids and sarcophagids emergence rate, increasing the mortality of pupae from intoxicated carcasses. Thus, this work demonstrates experimentally that Terbufos directly influences the development of flies with forensic potential and discusses the implications for PMI estimation, which can assist in future investigative processes with suspected poisoning by this organophosphate.
确定腐生性蝇类的发育时间被认为是估计死后时间（PMI）的一种准确方法。然而，杀虫剂和其他化学物质会改变蝇类的生命周期，导致 PMI 估计出现误差。因此，这项工作旨在评估不同剂量的特丁磷（有机磷）对与腐烂老鼠尸体相关的未成熟双翅目昆虫的时间分布模式和发育的影响。为此，将 150 克雌性 Wistar 大鼠通过灌胃接受 200 µl 的特丁磷（5 或 10 mg/kg）或蒸馏水（对照），给药 30 分钟后，将动物安乐死并分在悬挂的陷阱中，在环境条件下分解。每天收集分散的幼虫，并监测其发育时间，直到成年蝇出现。数据分析后发现，特丁磷改变了 1）幼虫分散的时间模式；2）定殖组合的组成和结构（出现的成虫）；3）物种的发育时间，根据使用的剂量加速或延迟其周期；4）加利福尼亚蝇和麻蝇的出现率，增加了中毒尸体中蛹的死亡率。因此，这项工作通过实验证明了特丁磷直接影响具有法医潜力的蝇类的发育，并讨论了对 PMI 估计的影响，这有助于未来对疑似这种有机磷中毒的调查过程。
