Laboratory of Insect and Vectors, Department of Microbiology and Parasitology, Biosciences Center, Federal University of Rio Grande do Norte, Natal, Rio Grande do Norte, Brazil.
Robotics and Machine Learning Team, Department of Mechanical Engineering, Federal University of Rio Grande do Norte, Natal, Rio Grande do Norte, Brazil.
Neotrop Entomol. 2023 Dec;52(6):1155-1164. doi: 10.1007/s13744-023-01094-6. Epub 2023 Oct 20.
Neurotoxicant compounds interfere with the behaviour and biology of insects, significantly altering their locomotion patterns. However, little is known about the effect of organophosphates, neurotoxicants for agricultural, domestic and industrial use, on the larval movement of necrophagous flies, although being responsible for frequent cases of poisoning and accidental or intentional deaths. Thus, we aimed to study the influence of Terbufos (organophosphate) on the activity and mobility patterns of Lucilia eximia (Wiedemann 1819) (Calliphoridae) and Peckia (Peckia) chrysostoma (Wiedemann 1830) (Sarcophagidae) immatures collected from rat carcasses intoxicated with 5, 10 or 20 mg/kg of Terbufos, to evaluate (i) peristaltic movements and body contractions, and (ii) distance and shape of the trajectory travelled by the larva. Behavioural parameters were analysed in loco and through videos. We observed that the presence of Terbufos altered poisoned larvae's activity and body mobility in both taxon and dose-dependent manner. Lucilia eximia larvae were more active, with greater frequency of body movements and lateral contractions when intoxicated with high and intermediate doses of Terbufos. On the other hand, P. (P.) chrysostoma immatures were less active, with fewer body and lateral contractions when intoxicated with the high dose of the compound. This work experimentally demonstrates that the presence of Terbufos can alters the mobility and movement of intoxicated necrophagous Diptera, essential components of the cadaveric fauna.
神经毒物化合物会干扰昆虫的行为和生物学，显著改变它们的运动模式。然而，对于农业、家庭和工业用神经毒物有机磷化合物对腐生性蝇类幼虫运动的影响，人们知之甚少，尽管这些化合物是导致频繁中毒和意外或故意死亡的原因。因此，我们旨在研究特丁磷（有机磷）对从摄入 5、10 或 20mg/kg 特丁磷的中毒大鼠尸体中收集的丽蝇（Calliphoridae）的 Lucilia eximia（Wiedemann 1819）和麻蝇（Sarcophagidae）的 Peckia（Peckia）chrysostoma（Wiedemann 1830）幼虫的活动和移动模式的影响，以评估（i）蠕动运动和身体收缩，以及（ii）幼虫所走轨迹的距离和形状。行为参数在现场和通过视频进行分析。我们观察到，特丁磷的存在以分类群和剂量依赖的方式改变了中毒幼虫的活动和身体移动性。当摄入高剂量和中剂量的特丁磷时，丽蝇幼虫更活跃，身体运动和侧向收缩的频率更高。另一方面，当摄入高剂量的化合物时，P.（P.）chrysostoma 幼虫的活动减少，身体和侧向收缩减少。这项工作实验性地证明，特丁磷的存在可以改变中毒腐生性双翅目昆虫的移动性和运动性，这些昆虫是尸体动物群的重要组成部分。
