Department of Endodontics, Faculty of Dentistry, Atatürk University, Erzurum, Turkey.
Department of Medical Genetics, Medical Faculty, Atatürk University, Erzurum, Turkey.
Int Endod J. 2021 Jul;54(7):1016-1025. doi: 10.1111/iej.13493. Epub 2021 Mar 7.
To evaluate the effect of single nucleotide polymorphisms in the COMT, OPRM1, 5HT1A, 5HT2A and 5HTR3B genes on the intensity of postoperative pain following root canal treatment.
Ninety-five patients with mandibular and maxillary molar teeth diagnosed with symptomatic apical periodontitis and a level of preoperative pain greater than 50 on a 100 mm visual analogue scale (VAS) were included. Salivary DNA was collected from the participants and stored in Eppendorf tubes at -80 °C. Preoperative percussion pain values were recorded before the root canal treatment procedures. After completion of root canal treatment, the participants were given instructions to record their postoperative pain intensity levels at 24, 48 and 72 h, 5 days and 1 week after treatment, using the VAS. A second visit for the patients after seven days was planned to record their intensity levels of percussion pain on VAS. The percussion test was performed by tapping on the occlusal surface of the tooth with a blunt instrument. A QIAamp DNA Mini Kit was used to isolate DNA from saliva, and SNP Genotyping Analysis software version 1 was used to analyse the genotypes by calculating FAM and HEX signals. The Kruskal-Wallis and Mann-Whitney U-tests were used to evaluate pain intensity values amongst the genotypes, alleles, haplotypes and allele combinations. Nominal data (gender, intake and tooth number) were analysed using a Chi-square test. Bonferroni correction was performed. Thus, the significance level was set at 1.6% (P = 0.016), 2.5% (P = 0.025) and 1.25% (P = 0.0125) for genotype, allele and haplotype comparisons, respectively.
There was no significant difference amongst the genotypes and alleles in terms of pre- and postoperative pain intensity. There was no significant difference amongst the haplotypes formed for the COMT gene in terms of pain intensity. Additionally, there was no significant association between the allelic combination formed for 5HT1A + 5HT2A genes and the intensity of postoperative pain.
The findings indicate that none of the evaluated SNPs for COMT, OPRM1, 5HT1A, 5HT2A and 5HTR3B genes were associated with the intensity of postoperative pain.
评估 COMT、OPRM1、5HT1A、5HT2A 和 5HTR3B 基因中单核苷酸多态性对根管治疗后术后疼痛强度的影响。
纳入 95 例下颌和上颌磨牙因症状性根尖周炎和术前疼痛评分大于 50（100mm 视觉模拟评分）诊断为根尖周炎的患者。从参与者中采集唾液 DNA，并储存在 -80°C 的 Eppendorf 管中。在根管治疗前记录术前叩诊疼痛值。根管治疗完成后，嘱患者在治疗后 24、48 和 72 小时、5 天和 1 周时使用视觉模拟评分法记录术后疼痛强度。计划在 7 天后对患者进行第二次就诊，以记录 VAS 上的叩诊疼痛强度。用钝器械敲击牙齿的咬合面进行叩诊试验。用 QIAamp DNA Mini 试剂盒从唾液中提取 DNA，用 SNP Genotyping Analysis 软件版本 1 分析基因型，计算 FAM 和 HEX 信号。采用 Kruskal-Wallis 和 Mann-Whitney U 检验评估基因型、等位基因、单倍型和等位基因组合之间的疼痛强度值。采用卡方检验分析名义数据（性别、摄入和牙齿数）。进行 Bonferroni 校正。因此，基因型、等位基因和单倍型比较的显著性水平分别设定为 1.6%（P=0.016）、2.5%（P=0.025）和 1.25%（P=0.0125）。
在术前和术后疼痛强度方面，基因型和等位基因之间没有显著差异。COMT 基因形成的单倍型在疼痛强度方面没有显著差异。此外，5HT1A+5HT2A 基因形成的等位基因组合与术后疼痛强度之间没有显著关联。
研究结果表明，评估的 COMT、OPRM1、5HT1A、5HT2A 和 5HTR3B 基因中的 SNP 均与术后疼痛强度无关。
