Postgraduate Program, School of Dentistry, Fluminense Federal University, Niterói, Brazil.
Clinical Research Unit, Fluminense Federal University, Rio de Janeiro, Brazil.
Int Endod J. 2023 Sep;56(9):1077-1091. doi: 10.1111/iej.13946. Epub 2023 Jun 24.
This study aimed to investigate whether single-nucleotide polymorphisms (SNPs) in the genes encoding 5-HTR2A (5-Hydroxytryptamine (serotonin) receptor 2A) and MTNR1A (melatonin receptor 1A) may contribute to postoperative pain perception after root canal treatment. We hypothesised that SNPs in HTR2A and MTNR1A genes were associated with postoperative pain after root canal treatment.
This genetic cohort study enrolled patients with single-rooted teeth diagnosed with pulp necrosis and asymptomatic apical periodontitis before root canal treatment. Root canal treatment was performed in one session using a standardized protocol. Postoperative pain and tenderness were assessed using a visual analogue scale (recorded every day for 7 days and on the 14th and 30th days after root canal treatment). Genomic DNA was extracted from saliva and used to genotype the SNPs in HTR2A (rs4941573 and rs6313) and MTNR1A (rs6553010, rs6847693 and rs13140012) using real-time polymerase chain reaction. Genotypes were compared using univariate and multivariate Poisson regression with generalized estimating equations (p < .05).
In total, 108 patients were enrolled in this study. The SNPs rs6553010 (MTNR1A), rs4941573 and rs6313 (HTR2A) were associated with an increased risk of developing pain after root canal treatment (p < .05).
This study suggests that SNPs in HTR2A and MTNR1A influence pain response after root canal treatment.
本研究旨在探讨编码 5-羟色胺（5-HT）受体 2A（5-Hydroxytryptamine (serotonin) receptor 2A）和褪黑素受体 1A（melatonin receptor 1A）的基因中单核苷酸多态性（SNPs）是否与根管治疗后术后疼痛感知有关。我们假设 HTR2A 和 MTNR1A 基因中的 SNPs 与根管治疗后术后疼痛有关。
本遗传队列研究纳入了单根牙齿患者，这些患者在根管治疗前被诊断为牙髓坏死和无症状根尖周炎。根管治疗采用标准化方案一次性完成。使用视觉模拟量表（在根管治疗后第 7 天、第 14 天和第 30 天每天记录一次）评估术后疼痛和压痛。从唾液中提取基因组 DNA，使用实时聚合酶链反应对 HTR2A（rs4941573 和 rs6313）和 MTNR1A（rs6553010、rs6847693 和 rs13140012）的 SNPs 进行基因分型。使用单变量和多变量泊松回归与广义估计方程（p <.05）比较基因型。
本研究共纳入 108 例患者。SNP rs6553010（MTNR1A）、rs4941573 和 rs6313（HTR2A）与根管治疗后疼痛发生风险增加相关（p <.05）。
本研究表明 HTR2A 和 MTNR1A 中的 SNPs 影响根管治疗后的疼痛反应。
