Save Sight Institute, University of Sydney, Sydney, New South Wales, Australia ; and.
Sydney Eye Hospital, Sydney, New South Wales.
Retina. 2021 Sep 1;41(9):1986-1993. doi: 10.1097/IAE.0000000000003137.
To describe the novel observation of spontaneously migrating retinal cells from living donor surgical retinal explants that express progenitor cell markers in the absence of exogenous growth factors.
Surgical retinal explants were harvested from 5 consecutive patients undergoing 23 G pars plana vitrectomy for the management of rhegmatogenous detachment. During surgery, equatorial flap tears were trimmed with the vitreous cutter and aspirated. Excised tissue was then regurgitated into a syringe containing balanced salt solution and immediately transferred to tissue culture. Migrating cells subsequently underwent immunohistochemical staining and their characteristics were compared with those of a spontaneously immortalized Müller stem cell line.
Spontaneously migrating cells were observed from samples taken from all 5 patients from Day 2 to 10 after transfer to culture. These cells were found to express embryonic cell markers, including paired box 6 (Pax6), sex-determining region Y-box 2 (Sox-2), nestin, cone-rod homeobox, and cyclin-dependent kinase inhibitor 1B (p27Kip1) as well as proteins consistent with early or retained differentiation down the Müller cell lineage, including glial fibrillary acidic protein and glutamine synthetase.
After injury, the human equatorial retina is capable of spontaneously producing cells that demonstrate migration and that express progenitor cell markers. In addition, these cells express proteins consistent with Müller cell lineage. These initial observations support the assertion that the human retina may possess the potential for regeneration and that surgical retinal explants could also act as a ready source of retinal progenitor cells.
描述从活体供体手术性视网膜外植体中自发迁移的视网膜细胞的新观察结果，这些细胞在没有外源性生长因子的情况下表达祖细胞标记物。
从连续 5 例行 23G 经睫状体平坦部玻璃体切除术治疗孔源性视网膜脱离的患者中采集手术性视网膜外植体。在手术过程中，用玻璃体切割器修剪赤道瓣撕裂并抽吸。切除的组织随后被反流到含有平衡盐溶液的注射器中，并立即转移到组织培养中。随后对迁移细胞进行免疫组织化学染色，并将其特征与自发永生化 Müller 干细胞系进行比较。
从转移到培养后的第 2 天到第 10 天，从所有 5 名患者的样本中观察到自发迁移的细胞。这些细胞被发现表达胚胎细胞标记物，包括配对盒 6（Pax6）、性别决定区 Y 框 2（Sox-2）、巢蛋白、视锥杆同源盒和细胞周期蛋白依赖性激酶抑制剂 1B（p27Kip1），以及与 Müller 细胞谱系早期或保留分化一致的蛋白质，包括神经胶质纤维酸性蛋白和谷氨酰胺合成酶。
在损伤后，人赤道部视网膜能够自发产生表现出迁移和表达祖细胞标记物的细胞。此外，这些细胞表达与 Müller 细胞谱系一致的蛋白质。这些初步观察结果支持这样一种观点，即人类视网膜可能具有再生的潜力，并且手术性视网膜外植体也可以作为视网膜祖细胞的现成来源。
