大鼠肾动脉夹闭性高血压逆转后血液中血小板活化因子（1-O-烷基-2-乙酰基甘油磷酸胆碱）水平升高。

Increased levels of platelet-activating factor (1-O-alkyl-2-acetylglycerophosphocholine) in blood after reversal of renal clip hypertension in the rat.

McGowan H M, Vandongen R, Kelly L D, Hill K J

University Department of Medicine, Royal Perth Hospital, Western Australia.

Clin Sci (Lond). 1988 Apr;74(4):393-6. doi: 10.1042/cs0740393.

Abstract

This study analyses whole blood in acutely unclipped one-kidney, one-clip (1K, 1C) hypertensive rats for the presence of platelet-activating factor (PAF), a potent vasodilator and a putative mediator of the rapid blood pressure (BP) fall seen after unclipping. 2. Hypertensive 1K, 1C rats were anaesthetized and a carotid and jugular cannula were inserted for BP measurement and anaesthetic infusion respectively. After a stable level of anaesthesia was attained, the constrictive clip was removed and BP was recorded for 30 min. 3. Blood was drawn from the aorta directly into ice-cold acetone. The extract was analysed for PAF by a bioassay using 5-hydroxy-[14C]tryptamine-labelled platelets. 4. Rats which showed a BP fall had elevated levels of PAF [55 +/- 6 (SEM) pg/ml] (P less than 0.01). 5. This supports the hypothesis that activation of PAF biosynthesis may be a mechanism contributing to the fall in BP seen after unclipping the 1K, 1C hypertensive rat.

摘要

本研究分析了急性未夹闭的单肾单夹（1K，1C）高血压大鼠的全血，以检测血小板活化因子（PAF）的存在，PAF是一种强效血管舒张剂，也是未夹闭后快速血压（BP）下降的一种假定介质。2. 将高血压1K，1C大鼠麻醉，分别插入颈动脉和颈静脉插管用于测量血压和输注麻醉剂。达到稳定麻醉水平后，移除收缩夹并记录血压30分钟。3. 直接从主动脉将血液抽取到冰冷的丙酮中。通过使用5-羟基-[14C]色胺标记的血小板的生物测定法分析提取物中的PAF。4. 血压下降的大鼠PAF水平升高[55±6（SEM）pg/ml]（P<0.01）。5. 这支持了以下假设：PAF生物合成的激活可能是导致1K，1C高血压大鼠夹闭后血压下降的一种机制。