通过参与基于课程的研究经验，本科生进行功能基因组筛选，确定果蝇血细胞发育基因。
A functional genomics screen identifying blood cell development genes in Drosophila by undergraduates participating in a course-based research experience.
机构信息
Department of Molecular, Cell, and Developmental Biology, University of California, Los Angeles, Los Angeles, CA 90095, USA.
Biomedical Research Minor, University of California, Los Angeles, Los Angeles, CA 90095, USA.
出版信息
G3 (Bethesda). 2021 Jan 18;11(1). doi: 10.1093/g3journal/jkaa028.
Undergraduate students participating in the UCLA Undergraduate Research Consortium for Functional Genomics (URCFG) have conducted a two-phased screen using RNA interference (RNAi) in combination with fluorescent reporter proteins to identify genes important for hematopoiesis in Drosophila. This screen disrupted the function of approximately 3500 genes and identified 137 candidate genes for which loss of function leads to observable changes in the hematopoietic development. Targeting RNAi to maturing, progenitor, and regulatory cell types identified key subsets that either limit or promote blood cell maturation. Bioinformatic analysis reveals gene enrichment in several previously uncharacterized areas, including RNA processing and export and vesicular trafficking. Lastly, the participation of students in this course-based undergraduate research experience (CURE) correlated with increased learning gains across several areas, as well as increased STEM retention, indicating that authentic, student-driven research in the form of a CURE represents an impactful and enriching pedagogical approach.
参与加州大学洛杉矶分校本科生功能基因组学研究联盟（URCFG）的本科生使用 RNA 干扰（RNAi）与荧光报告蛋白相结合进行了两阶段筛选，以鉴定在果蝇中造血重要的基因。该筛选破坏了大约 3500 个基因的功能，并确定了 137 个候选基因，其功能丧失导致造血发育中出现可观察到的变化。将 RNAi 靶向成熟、祖细胞和调节细胞类型，确定了限制或促进血细胞成熟的关键亚群。生物信息学分析显示，基因在几个以前未被描述的领域中富集，包括 RNA 处理和输出以及囊泡运输。最后，学生在这种基于课程的本科生研究体验（CURE）中的参与与多个领域的学习收益增加以及 STEM 保留率增加相关，这表明以 CURE 形式的真实的、由学生驱动的研究代表了一种有影响力和丰富的教学方法。
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